Reference Report

Reference
Citation	Kiger, J.A., Natzle, J.E., Kimbrell, D.A., Paddy, M.R., Kleinhesselink, K., Green, M.M. (2007). Tissue remodeling during maturation of the Drosophila wing. Dev. Biol. 301(1): 178--191. (Export to RIS)
FlyBase ID	FBrf0192456
Publication Type	Research paper
PubMed ID	16962574
PubMed Abstract	The final step in morphogenesis of the adult fly is wing maturation, a process not well understood at the cellular level due to the impermeable and refractive nature of cuticle synthesized some 30 h prior to eclosion from the pupal case. Advances in GFP technology now make it possible to visualize cells using fluorescence after cuticle synthesis is complete. We find that, between eclosion and wing expansion, the epithelia within the folded wing begin to delaminate from the cuticle and that delamination is complete when the wing has fully expanded. After expansion, epithelial cells lose contact with each other, adherens junctions are disrupted, and nuclei become pycnotic. The cells then change shape, elongate, and migrate from the wing into the thorax. During wing maturation, the Timp gene product, tissue inhibitor of metalloproteinases, and probably other components of an extracellular matrix are expressed that bond the dorsal and ventral cuticular surfaces of the wing following migration of the cells. These steps are dissected using the batone and Timp genes and ectopic expression of alphaPS integrin, inhibitors of Armadillo/beta-catenin nuclear activity and baculovirus caspase inhibitor p35. We conclude that an epithelial-mesenchymal transition is responsible for epithelial delamination and dissolution.
DOI	10.1016/j.ydbio.2006.08.011
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (1)
	Df(3R)Timp-Syn²⁸
Alleles (18)
	arm^{cMa.T:Avic\GFP} arm^{S2.Scer\UAS.T:Hsap\MYC} arm^{S10.Scer\UAS.T:Hsap\MYC} arm^{Δ.Scer\UAS.T:Ivir\HA1} Avic\GFP^{Scer\UAS.T:SV40\nls2} Avic\GFP^y.wing BacA\p35^Scer\UAS.cHa bae¹ Disc\RFP^Scer\UAS.cKa if^m8.Scer\UAS pygo^Scer\UAS.cPa Scer\GAL4^30A Scer\GAL4⁶⁸⁴ Scer\GAL4^ap-md544 Scer\GAL4^He.PZ sgg^Scer\UAS.cBa Syn²⁸ Timp²⁸
Constructs (12)
	P{arm-GFP} P{He-GAL4.Z} P{UAS-arm.S2} P{UAS-arm.S10} P{UAS-arm.Δ.flu} P{UAS-AUG-DsRed} P{UAS-GFP.nls} P{UAS-if.m8} P{UAS-p35.H} P{UAS-pygo.P} P{UAS-sgg.B} P{y-GFP.wing}
Genes (14)
	ap arm Avic\GFP BacA\p35 bae Disc\RFP He if mew pygo Scer\GAL4 sgg Syn Timp
Insertions (3)
	P{GAL4}684 P{GawB}30A P{GawB}ap^md544