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Jenny, A., Hachet, O., Zavorszky, P., Cyrklaff, A., Weston, M.D.J., Johnston, D.S., Erdelyi, M., Ephrussi, A. (2006). A translation-independent role of oskar RNA in early Drosophila oogenesis.  Development 133(15): 2827--2833.
FlyBase ID
FBrf0192546
Publication Type
Research paper
Abstract

The Drosophila maternal effect gene oskar encodes the posterior determinant responsible for the formation of the posterior pole plasm in the egg, and thus of the abdomen and germline of the future fly. Previously identified oskar mutants give rise to offspring that lack both abdominal segments and a germline, thus defining the ;posterior group phenotype'. Common to these classical oskar alleles is that they all produce significant amounts of oskar mRNA. By contrast, two new oskar mutants in which oskar RNA levels are strongly reduced or undetectable are sterile, because of an early arrest of oogenesis. This egg-less phenotype is complemented by oskar nonsense mutant alleles, as well as by oskar transgenes, the protein-coding capacities of which have been annulled. Moreover, we show that expression of the oskar 3' untranslated region (3'UTR) is sufficient to rescue the egg-less defect of the RNA null mutant. Our analysis thus reveals an unexpected role for oskar RNA during early oogenesis, independent of Oskar protein. These findings indicate that oskar RNA acts as a scaffold or regulatory RNA essential for development of the oocyte.

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PubMed Central ID
Related Publication(s)
Note

What is so special about oskar wild?
O'Gorman and Akoulitchev, 2006, Sci. STKE 2006(365): pe51 [FBrf0193299]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Development
    Title
    Development
    Publication Year
    1987-
    ISBN/ISSN
    0950-1991
    Data From Reference
    Aberrations (1)
    Alleles (16)
    Genes (7)
    Natural transposons (3)
    Insertions (2)
    Experimental Tools (1)
    Transgenic Constructs (11)