Reference Report

Reference
Citation	Srahna, M., Leyssen, M., Choi, C.M., Fradkin, L.G., Noordermeer, J.N., Hassan, B.A. (2006). A signaling network for patterning of neuronal connectivity in the Drosophila brain. PLoS Biol. 4(11): e348. (Export to RIS)
FlyBase ID	FBrf0192599
Publication Type	Research paper
PubMed ID	17032066
PubMed Abstract	The precise number and pattern of axonal connections generated during brain development regulates animal behavior. Therefore, understanding how developmental signals interact to regulate axonal extension and retraction to achieve precise neuronal connectivity is a fundamental goal of neurobiology. We investigated this question in the developing adult brain of Drosophila and find that it is regulated by crosstalk between Wnt, fibroblast growth factor (FGF) receptor, and Jun N-terminal kinase (JNK) signaling, but independent of neuronal activity. The Rac1 GTPase integrates a Wnt-Frizzled-Disheveled axon-stabilizing signal and a Branchless (FGF)-Breathless (FGF receptor) axon-retracting signal to modulate JNK activity. JNK activity is necessary and sufficient for axon extension, whereas the antagonistic Wnt and FGF signals act to balance the extension and retraction required for the generation of the precise wiring pattern.
DOI	10.1371/journal.pbio.0040348
Related Publication(s)
Review	Signals that tell fly neurons to extend or retract. Chanut, 2006, PLoS Biol. 4(11): e391 [FBrf0200120]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	PLoS Biol.
Title	PLoS Biology
Publication Year	2003-
ISBN/ISSN	1545-7885 1544-9173
Data from Reference
Alleles (40)
	Axn^Scer\UAS.cHa bsk² bsk^DN.Scer\UAS btl^DN.Scer\UAS btl^H852-3 btl^Scer\UAS.cDa Cdc42^N17.Scer\UAS Cdc42^V12.Scer\UAS dsh¹ dsh⁶ Ecol\lacZ^bnl-06916 Ecol\lacZ^puc-A251.1F3 Ecol\lacZ^Scer\UAS.cUa Ecol\lacZ^{Scer\UAS.O.T:Btau\MAPT} Egfr^DN.Scer\UAS fz2^C1 fz2^e4 fz2^Scer\UAS.N fz3^G10 fz¹⁵ hep¹ hep^CA.Scer\UAS hep^Scer\UAS.cBa Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Mtl^Δ n-syb^{Scer\UAS.cEa.T:Avic\GFP} Ork1^{Δ-C.Scer\UAS.T:Avic\GFP-EGFP} Rac1^J10 Rac1^J11 Rac1^N17.Scer\UAS Rac1^Scer\UAS.cLa Rac1^V12.Scer\UAS Rac2^Δ Rho1^N19.Scer\UAS Rho1^V14.Scer\UAS Scer\GAL4^ato.3.6 shi^{K44A.Scer\UAS} Wnt2^O Wnt4^EMS23 Wnt5⁴⁰⁰
Constructs (22)
	P{ato-GAL4.3.6} P{DN-R1} P{UAS-Axn.H} P{UAS-bsk.DN} P{UAS-btl.D} P{UAS-Cdc42.N17} P{UAS-Cdc42.V12} P{UAS-Egfr.DN} P{UAS-fz2.N} P{UAS-hep.B} P{UAS-hep.CA} P{UAS-lacZ.btau.YES} P{UAS-lacZ.U} P{UAS-mCD8::GFP.L} P{UAS-n-syb-GFP.E} P{UAS-Ork1.Δ-C} P{UAS-Rac1.L} P{UAS-Rac1.N17} P{UAS-Rac1.V12} P{UAS-Rho1.N19} P{UAS-Rho1.V14} P{UAS-shi.K44A}
Genes (27)
	Axn bnl bsk btl Cdc42 dsh Ecol\lacZ Egfr fz fz2 fz3 hep Mmus\Cd8a Mtl n-syb Ork1 puc Rac1 Rac2 Rho1 Scer\GAL4 shi sty wg Wnt2 Wnt4 Wnt5
Insertions (8)
	P{ato-GAL4.3.6}14a P{lArB}puc^A251.1F3 P{PZ}bnl⁰⁶⁹¹⁶ P{UAS-Cdc42.N17}3 P{UAS-Ork1.Δ-C}1 P{UAS-Ork1.Δ-C}2 P{UAS-shi.K44A}3-7 P{UAS-shi.K44A}3-10