Reference Report

Reference
Citation	Bidla, G., Dushay, M.S., Theopold, U. (2007). Crystal cell rupture after injury in Drosophila requires the JNK pathway, small GTPases and the TNF homolog Eiger. J. Cell Sci. 120(7): 1209--1215. (Export to RIS)
FlyBase ID	FBrf0192653
Publication Type	Research paper
PubMed ID	17356067
PubMed Abstract	The prophenoloxidase-activating cascade is a key component of arthropod immunity. Drosophila prophenoloxidase is stored in crystal cells, a specialized class of blood cells from which it is released through cell rupture. Within minutes after bleeding, prophenoloxidase is activated leading to visible melanization of the clot matrix. Using crystal cell rupture and melanization as readouts to screen mutants in signal transduction pathways, we show that prophenoloxidase release requires Jun N-terminal kinase, small Rho GTPases and Eiger, the Drosophila homolog of tumor necrosis factor. We also provide evidence that in addition to microbial products, endogenous signals from dying hemocytes contribute to triggering and/or assembly of the prophenoloxidase-activating cascade, and that this process can be inhibited in vitro and in vivo using the viral apoptotic inhibitor p35. Our results provide a more comprehensive view of immune signal transduction pathways, with implications for immune reactions where cell death is used as a terminal mode of cell activation.
DOI	10.1242/jcs.03420
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Sci.
Title	Journal of Cell Science
Publication Year	1966-
ISBN/ISSN	0021-9533
Data from Reference
Aberrations (1)
	Df(2R)stan1
Alleles (26)
	Avic\GFP^{S65T.Scer\UAS} BacA\p35^Scer\UAS.cHa Bc¹ bsk^{dsRNA.Scer\UAS} Ecol\lacZ^puc-A251.1F3 egr¹ egr³ egr^Scer\UAS.cIa GNBP1^e03371 grim^Scer\UAS.cNa hep^NIG.4353R Hml^f03374 lz^r15 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} PGRP-LC^ΔE PGRP-LE¹¹² PGRP-SA^seml Rho1^V14.Scer\UAS Scer\GAL4^Act5C.PU Scer\GAL4^da.G32 Scer\GAL4^He.PZ Scer\GAL4^Lsp2.PH Scer\GAL4^lz-gal4 Sp7^{dsRNA.Scer\UAS} Spn27A^Scer\UAS.cLa spz²
Constructs (14)
	P{Act5C-GAL4.U} P{GAL4-da.G32} P{He-GAL4.Z} P{Lsp2-GAL4.H} P{NIG.4353R} P{UAS-bsk.IR} P{UAS-eiger.I} P{UAS-GFP.S65T} P{UAS-grim.N} P{UAS-mCD8::GFP.L} P{UAS-p35.H} P{UAS-Rho1.V14} P{UAS-Sp7.dsRNA} P{UAS-Spn27A.L}
Genes (24)
	Avic\GFP BacA\p35 Bc bsk Ecol\lacZ egr GNBP1 grim He hep Hml lz Mmus\Cd8a PGRP-LC PGRP-LE PGRP-SA puc Rel Rho1 Scer\GAL4 Sp7 Spn27A spz Tak1
Insertions (2)
	P{GawB}lz^gal4 P{lArB}puc^A251.1F3