Reference Report

Reference
Citation	Benton, R., Sachse, S., Michnick, S.W., Vosshall, L.B. (2006). Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. PLoS Biol. 4(2): e20. (Export to RIS)
FlyBase ID	FBrf0192657
Publication Type	Research paper
PubMed ID	16402857
PubMed Abstract	Drosophila olfactory sensory neurons (OSNs) each express two odorant receptors (ORs): a divergent member of the OR family and the highly conserved, broadly expressed receptor OR83b. OR83b is essential for olfaction in vivo and enhances OR function in vitro, but the molecular mechanism by which it acts is unknown. Here we demonstrate that OR83b heterodimerizes with conventional ORs early in the endomembrane system in OSNs, couples these complexes to the conserved ciliary trafficking pathway, and is essential to maintain the OR/OR83b complex within the sensory cilia, where odor signal transduction occurs. The OR/OR83b complex is necessary and sufficient to promote functional reconstitution of odor-evoked signaling in sensory neurons that normally respond only to carbon dioxide. Unexpectedly, unlike all known vertebrate and nematode chemosensory receptors, we find that Drosophila ORs and OR83b adopt a novel membrane topology with their N-termini and the most conserved loops in the cytoplasm. These loops mediate direct association of ORs with OR83b. Our results reveal that OR83b is a universal and integral part of the functional OR in Drosophila. This atypical heteromeric and topological design appears to be an insect-specific solution for odor recognition, making the OR/OR83b complex an attractive target for the development of highly selective insect repellents to disrupt olfactory-mediated host-seeking behaviors of insect disease vectors.
DOI	10.1371/journal.pbio.0040020
Related Publication(s)
Review	A novel design principle for the insect odorant receptor. Gross, 2006, PLoS Biol. 4(2): e34 [FBrf0200116]
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	PLoS Biol.
Title	PLoS Biology
Publication Year	2003-
ISBN/ISSN	1545-7885 1544-9173
Data from Reference
Aberrations (2)
	Df(2L)dp-79b Dp(2;2)dpp^d21
Alleles (41)
	Avic\GFP^{GCaMP1.3.Scer\UAS.cAa} Avic\GFP^{YFP(1).ZIP.Scer\UAS} Avic\GFP^{YFP(2).ZIP.Scer\UAS} Gr21a::Orco^{Scer\UAS.T:Avic\GFP-EGFP} Gr21a^{Scer\UAS.T:Avic\GFP-EGFP} Gr21a^{Scer\UAS.T:Avic\GFP-YFP(1)} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} ninaE^{Nterm.MtnA.T:Ecol\lacZ,T:Zzzz\TM} ninaE^{Nterm.MtnA.T:Ecol\lacZ} ninaE^{Nterm.TM1.MtnA.T:Ecol\lacZ,T:Zzzz\TM} ninaE^{Nterm.TM1.MtnA.T:Ecol\lacZ} Or9a^{Nterm.MtnA.T:Ecol\lacZ,T:Zzzz\TM} Or9a^{Nterm.MtnA.T:Ecol\lacZ} Or9a^{Nterm.TM1.MtnA.T:Ecol\lacZ,T:Zzzz\TM} Or9a^{Nterm.TM1.MtnA.T:Ecol\lacZ} Or43a^{Scer\UAS.T:Avic\GFP-EGFP} Or43a^{Scer\UAS.T:Avic\GFP-YFP(1)} Or43a^{Scer\UAS.T:Avic\GFP-YFP(2)} Or43a^{Scer\UAS.YFP(1).ZIP} Or43a^{Scer\UAS.YFP(2).ZIP} Orco¹ Orco² Orco^{Nterm.MtnA.T:Ecol\lacZ,T:Zzzz\TM} Orco^{Nterm.MtnA.T:Ecol\lacZ} Orco^{Nterm.TM1.MtnA.T:Ecol\lacZ,T:Zzzz\TM} Orco^{Nterm.TM1.MtnA.T:Ecol\lacZ} Orco^Scer\UAS.cBa Orco^{Scer\UAS.T:Avic\GFP-EGFP} Orco^{Scer\UAS.T:Avic\GFP-YFP(1)} Orco^{Scer\UAS.T:Avic\GFP-YFP(2)} Orco^{Scer\UAS.YFP(1).ZIP} Orco^{Scer\UAS.YFP(2).ZIP} Scer\GAL4^AB1 Scer\GAL4^Gr21a.9.323 Scer\GAL4^Or22a.7.717 Scer\GAL4^Or47b.7.467 Scer\GAL4^{Orco.2.642.T:Hsim\VP22} Scer\GAL4^osm-1.PA-R Scer\GAL80^ts.αTub84B T:Avic\GFP^EGFP T:Avic\GFP^YFP
Constructs (23)
	P{Or22a-GAL4.7.717} P{Or47b-GAL4.7.467} P{Orco-GAL4.W} P{osm-1-GAL4.A} P{tubP-GAL80^ts} P{UAS-GCaMP1.3.A} P{UAS-Gr21a::Or83b.EGFP} P{UAS-Gr21a.EGFP} P{UAS-Gr21a.YFP(1)} P{UAS-mCD8::GFP.L} P{UAS-Or43a.EGFP} P{UAS-Or43a.YFP(1).ZIP} P{UAS-Or43a.YFP(1)} P{UAS-Or43a.YFP(2).ZIP} P{UAS-Or43a.YFP(2)} P{UAS-Orco.B} P{UAS-Orco.EGFP} P{UAS-Orco.YFP(1).ZIP} P{UAS-Orco.YFP(1)} P{UAS-Orco.YFP(2).ZIP} P{UAS-Orco.YFP(2)} P{UAS-YFP(1).ZIP} P{UAS-YFP(2).ZIP}
Genes (15)
	Avic\GFP Gr21a Gr21a::Orco Mmus\Cd8a ninaE Or9a Or22a Or22b Or43a Orco Scer\GAL4 Scer\GAL80 T:Ecol\lacZ T:Scer\GCN4 T:Zzzz\TM
Insertions (1)
	P{GawB}AB1
Natural transposons (1)
	P-element