Reference Report

Reference
Citation	Tsruya, R., Wojtalla, A., Carmon, S., Yogev, S., Reich, A., Bibi, E., Merdes, G., Schejter, E., Shilo, B.Z. (2007). Rhomboid cleaves Star to regulate the levels of secreted Spitz. EMBO J. 26(5): 1211--1220. (Export to RIS)
FlyBase ID	FBrf0192747
Publication Type	Research paper
PubMed ID	17304216
PubMed Abstract	Intracellular trafficking of the precursor of Spitz (Spi), the major Drosophila EGF receptor (EGFR) ligand, is facilitated by the chaperone Star, a type II transmembrane protein. This study identifies a novel mechanism for modulating the activity of Star, thereby influencing the levels of active Spi ligand produced. We demonstrate that Star can efficiently traffic Spi even when present at sub-stoichiometric levels, and that in Drosophila S(2)R(+) cells, Spi is trafficked from the endoplasmic reticulum to the late endosome compartment, also enriched for Rhomboid, an intramembrane protease. Rhomboid, which cleaves the Spi precursor, is now shown to also cleave Star within its transmembrane domain both in cell culture and in flies, expanding the repertoire of known Rhomboid substrates to include both type I and type II transmembrane proteins. Cleavage of Star restricts the amount of Spi that is trafficked, and may explain the exceptional dosage sensitivity of the Star locus in flies.
DOI	10.1038/sj.emboj.7601581
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	EMBO J.
Title	The EMBO Journal
Publication Year	1982-
ISBN/ISSN	0261-4189
Data from Reference
Alleles (8)
	Ecol\lacZ^LL rho^Scer\UAS.cTa ru^Scer\UAS.cTa S^{C1.Scer\UAS.T:Ecol\lexA,T:Hsim\VP16} S^{Scer\UAS.T:Ecol\lexA,T:Hsim\VP16} Scer\GAL4^GMR.PF Scer\GAL4^prd.RG1 w^+mC
Constructs (7)
	P{GAL4-ninaE.GMR} P{GAL4-prd.F} P{LL.lacZ} P{UAS-rho1.T} P{UAS-rho3.T} P{UAS-S.C1-LV} P{UAS-S.LV}
Genes (14)
	Ecol\lacZ Glg1 Hsc70-3 Rab5 Rab7 Rab11 rho ru S Scer\GAL4 spi T:Ecol\lexA T:Hsim\VP16 w
Natural transposons (1)
	P-element