Reference Report

Reference
Citation	Yoshioka, Y., Suyari, O., Yamada, M., Ohno, K., Hayashi, Y., Yamaguchi, M. (2007). Complex interference in the eye developmental pathway by Drosophila NF-YA. genesis 45(1): 21--31. (Export to RIS)
FlyBase ID	FBrf0192776
Publication Type	Research paper
PubMed ID	17216611
PubMed Abstract	The CCAAT motif-binding factor NF-Y consists of three different subunits, NF-YA, NF-YB, and NF-YC, all of which are required for formation of the NF-Y complex and DNA-binding. NF-YA contains a DNA binding domain in its C-terminal region. We established transgenic fly lines carrying the UAS-HA-dNF-YA or UAS-dNF-YAIR and showed over-expression or knockdown with various GAL4 drivers to be lethal at various developmental stages, suggesting that dNF-YA participate in various gene regulatory pathways during Drosophila development. Expression of dNF-YA with eyeless-GAL4 mainly resulted in lethality with a headless phenotype in pharate-adults. Reduction of the eyeless gene dose enhanced the dNF-YA-induced phenotype, while reduction of the Distal-less gene dose suppressed the phenotype. On the other hand, crossing the dNF-YA over-expressing flies with Notch mutant resulted in no apparent effect on the phenotype. These results suggest that dNF-YA can disturb eye disc specification, but not eye disc growth.
DOI	10.1002/dvg.20260
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Language of Publication	English
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Publication Type	Journal
Abbreviation	genesis
Title	genesis
Publication Year	2000-
ISBN/ISSN	1526-954X 1526-968X
Data from Reference
Alleles (17)
	Dll⁵ Dll⁹ ey¹ ey² ey⁴ ey^Scer\UAS.cHa N^264-39 Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ} Nf-YA^{Scer\UAS.T:Ivir\HA1} Scer\GAL4^69B Scer\GAL4^91Y Scer\GAL4^Act5C.PU Scer\GAL4^Bx-MS1096 Scer\GAL4^en-e16E Scer\GAL4^ey.PH Scer\GAL4^GMR.PS w^+mC
Constructs (6)
	P{Act5C-GAL4.U} P{GAL4-ey.H} P{GAL4-GMR.S} P{UAS-ey.H} P{UAS-Nf-YA.HA} P{UAS-Nf-YA.WIZ.231-399}
Genes (12)
	ct Dll elav ey eya N Nf-YA Nf-YB Nf-YC Scer\GAL4 T:Ivir\HA1 w
Insertions (4)
	P{en2.4-GAL4}e16E P{GawB}69B P{GawB}91Y^91Y P{GawB}Bx^MS1096