Reference Report

Reference
Citation	Larsen, C., Franch-Marro, X., Hartenstein, V., Alexandre, C., Vincent, J.P. (2006). An efficient promoter trap for detection of patterned gene expression and subsequent functional analysis in Drosophila. Proc. Natl. Acad. Sci. U.S.A. 103(47): 17813--17817. (Export to RIS)
FlyBase ID	FBrf0192951
Publication Type	Research paper
PubMed ID	17093046
PubMed Abstract	Transposable elements have been used in Drosophila to detect gene expression, inactivate gene function, and induce ectopic expression or overexpression. We have combined all of these features in a single construct. A promoterless GAL4 cDNA is expressed when the construct inserts within a transcriptional unit, and GAL4 activates a GFP-encoding gene present in the same transposon. In a primary screen, patterned gene expression is detected as GFP fluorescence in the live progeny of dysgenic males. Many animals expressing GFP in distinct patterns can be recovered with relatively little effort. As expected, many insertions cause loss of function. After insertion at a genomic location, specific parts of the transposon can be excised by FLP recombinase, thus allowing it to induce conditional misexpression of the tagged gene. Therefore, both gain- and loss-of-function studies can be carried out with a single insertion in a gene identified by virtue of its expression pattern. Using this promoter trap approach, we have identified a group of cells that innervate the calyx of the mushroom body and could thus define a previously unrecognized memory circuit.
DOI	10.1073/pnas.0607652103
Related Publication(s)
Supplementary material	Supporting Table 2. [FBrf0202556]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (22)
	Avic\GFP³⁴ Avic\GFP³⁵ Avic\GFP⁵⁶ Avic\GFP⁸⁶ Avic\GFP⁹⁰ Avic\GFP⁹⁸ Avic\GFP^CG6301-PT1 Avic\GFP^Csk-50 Avic\GFP^elav-95 Avic\GFP^Mhc-PT1 Avic\GFP^{Scer\UAS.FRT1} elav⁹⁵ elav^FO Mhc^FO Mhc^PT1 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^CG6301-PT1 Scer\GAL4^elav-95 Scer\GAL4^en-e16E Scer\GAL4^FRT2 Scer\GAL4^Mhc-PT1 w^+mC
Constructs (4)
	P{PT1} P{PT1Δ} P{PT2} P{UAS-mCD8::GFP.L}
Genes (14)
	Avic\GFP CadN CG34459 CG34460 cpo elav L MESK2 Mhc Mmus\Cd8a odd Sam-S Scer\GAL4 w
Insertions (14)
	P{en2.4-GAL4}e16E P{PT1}34 P{PT1}35 P{PT1}56 P{PT1}86 P{PT1}90 P{PT1}98 P{PT1}CG6301^PT1 P{PT1}Csk⁵⁰ P{PT1}elav⁹⁵ P{PT1}Mhc^PT1 P{PT1Δ}CG6301^FO P{PT1Δ}elav^FO P{PT1Δ}Mhc^FO
Natural transposons (1)
	P-element