Reference Report

Reference
Citation	Ghabrial, A.S., Krasnow, M.A. (2006). Social interactions among epithelial cells during tracheal branching morphogenesis. Nature 441(7094): 746--749. (Export to RIS)
FlyBase ID	FBrf0192962
Publication Type	Research paper
PubMed ID	16760977
PubMed Abstract	Many organs are composed of tubular networks that arise by branching morphogenesis in which cells bud from an epithelium and organize into a tube. Fibroblast growth factors (FGFs) and other signalling molecules have been shown to guide branch budding and outgrowth, but it is not known how epithelial cells coordinate their movements and morphogenesis. Here we use genetic mosaic analysis in Drosophila melanogaster to show that there are two functionally distinct classes of cells in budding tracheal branches: cells at the tip that respond directly to Branchless FGF and lead branch outgrowth, and trailing cells that receive a secondary signal to follow the lead cells and form a tube. These roles are not pre-specified; rather, there is competition between cells such that those with the highest FGF receptor activity take the lead positions, whereas those with less FGF receptor activity assume subsidiary positions and form the branch stalk. Competition appears to involve Notch-mediated lateral inhibition that prevents extra cells from assuming the lead. There may also be cooperation between budding cells, because in a mosaic epithelium, cells that cannot respond to the chemoattractant, or respond only poorly, allow other cells in the epithelium to move ahead of them.
DOI	10.1038/nature04829
Related Publication(s)
Review	In migration, it's lead, follow, or get out of the way. Kruger, 2006, Cell 125(7): 1211 [FBrf0198691]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nature
Title	Nature
Publication Year	1869-
ISBN/ISSN	0028-0836
Data from Reference
Alleles (20)
	Avic\GFP^{dsRNA.i.hp.Scer\UAS} Avic\GFP^Scer\UAS.cUa btl⁷²⁴ btl⁷⁸⁸ btl¹¹¹⁸ btl¹¹⁸⁷ btl¹⁴⁷⁶ btl¹⁶⁸⁴ btl^BN btl^LG18 Disc\RFP^{DsRed2.Scer\UAS.T:Uuuu\nls6} Disc\RFP^Scer\UAS.cGa N^ICN.Scer\UAS N^l1N-ts1 pnt¹⁹⁸ pnt¹³¹⁸ pnt^unspecified pnt^Δ88 Scer\GAL4^btl.PS stumps^unspecified
Constructs (6)
	P{GAL4-btl.S} P{UAS-DsRed2.nls} P{UAS-DsRed.G} P{UAS-GFP.U} P{UAS-iGFP.hp} P{UAS-N.ICN}
Genes (8)
	Avic\GFP btl Disc\RFP N pnt Scer\GAL4 stumps T:Uuuu\nls6
Natural transposons (1)
	P-element