Reference Report

Reference
Citation	Legent, K., Dutriaux, A., Delanoue, R., Silber, J. (2006). Cell cycle genes regulate vestigial and scalloped to ensure normal proliferation in the wing disc of Drosophila melanogaster. Genes Cells 11(8): 907--918. (Export to RIS)
FlyBase ID	FBrf0193006
Publication Type	Research paper
PubMed ID	16866874
PubMed Abstract	In Drosophila, the Vestigial-Scalloped (VG-SD) dimeric transcription factor is required for wing cell identity and proliferation. Previous results have shown that VG-SD controls expression of the cell cycle positive regulator dE2F1 during wing development. Since wing disc growth is a homeostatic process, we investigated the possibility that genes involved in cell cycle progression regulate vg and sd expression in feedback loops. We focused our experiments on two major regulators of cell cycle progression: dE2F1 and the antagonist dacapo (dap). Our results reinforce the idea that VG/SD stoichiometry is critical for correct development and that an excess in SD over VG disrupts wing growth. We reveal that transcriptional activity of VG-SD and the VG/SD ratio are both modulated by down-expression of cell cycle genes. We also detected a dap-induced sd up-regulation that disrupts wing growth. Moreover, we observed a rescue of a vg hypomorphic mutant phenotype by dE2F1 that is concomitant with vg and sd induction. This regulation of the VG-SD activity by dE2F1 is dependent on the vg genetic background. Our results support the hypothesis that cell cycle genes fine-tune wing growth and cell proliferation, in part, through control of the VG/SD stoichiometry and activity. This points to a homeostatic feedback regulation between proliferation regulators and the VG-SD wing selector.
DOI	10.1111/j.1365-2443.2006.00993.x
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genes Cells
Title	Genes to cells : devoted to molecular & cellular mechanisms
Publication Year	1996-
ISBN/ISSN	1356-9597
Data from Reference
Alleles (20)
	Avic\GFP^Scer\UAS.cUa BacA\p35^Scer\UAS.cHa dap⁰⁴⁴⁵⁴ dap^Scer\UAS.cLa Dp^Scer\UAS.cDa E2f⁹¹ E2f^Scer\UAS.cNa Ecol\lacZ^Scer\UAS.cUa Ecol\lacZ^sd-ETX4 Ecol\lacZ^vg.806 Ecol\lacZ^vg.int2.1 Scer\GAL4^ptc-559.1 Scer\GAL4^vg.int2.1 sd¹ sd^58d sd^{213-440.hs.T:Scer\GAL4} sd^Scer\UAS.cVa vg^83b27 vg^null vg^Scer\UAS.cZa
Constructs (12)
	P{hs-sd.T:Scer\GAL4} P{UAS-dap.L} P{UAS-Dp.D} P{UAS-E2f.N} P{UAS-GFP.U} P{UAS-lacZ.U} P{UAS-p35.H} P{UAS-sd.V} P{UAS-vg.Z} P{vg(806)-lacZ} P{vg(D/V)-lacZ} P{vg(int2.1)-GAL4}
Genes (12)
	Avic\GFP BacA\p35 ct dap Dp E2f Ecol\lacZ His3 ptc Scer\GAL4 sd vg
Insertions (2)
	P{GawB}ptc^559.1 P{lArB}sd^ETX4