Reference Report

Reference
Citation	Secombe, J., Li, L., Carlos, L., Eisenman, R.N. (2007). The Trithorax group protein Lid is a trimethyl histone H3K4 demethylase required for dMyc-induced cell growth. Genes Dev. 21(5): 537--551. (Export to RIS)
FlyBase ID	FBrf0193047
Publication Type	Research paper
PubMed ID	17311883
PubMed Abstract	The Myc oncoprotein is a potent inducer of cell growth, cell cycle progression, and apoptosis. While many direct Myc target genes have been identified, the molecular determinants of Myc's transcriptional specificity remain elusive. We have carried out a genetic screen in Drosophila and identified the Trithorax group protein Little imaginal discs (Lid) as a regulator of dMyc-induced cell growth. Lid binds to dMyc and is required for dMyc-induced expression of the growth regulatory gene Nop60B. The mammalian Lid orthologs, Rbp-2 (JARID1A) and Plu-1 (JARID1B), also bind to c-Myc, indicating that Lid-Myc function is conserved. We demonstrate that Lid is a JmjC-dependent trimethyl H3K4 demethylase in vivo and that this enzymatic activity is negatively regulated by dMyc, which binds to Lid's JmjC domain. Because Myc binding is associated with high levels of trimethylated H3K4, we propose that the Lid-dMyc complex facilitates Myc binding to, or maintenance of, this chromatin context.
DOI	10.1101/gad.1523007
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Aberrations (9)
	Df(1)C246 Df(2L)BSC5 Df(2L)cl-h3 Df(2L)E110 Df(2L)net-PMF Df(3L)H99 Df(3R)DG2 Df(3R)SR29 T(1;3)sc^J4
Alleles (32)
	ago⁴ ash1^B1 ash2¹ Asx^XF23 brm² Cdk4^Scer\UAS.cMa CycD^Scer\UAS.cDa CycE^AR95 dap^2X10 dm^P0 dm^Scer\UAS.cZa E2f^unspecified E(Pc)¹ Gcn5^unspecified kis¹ lid^17.1 lid¹⁰⁴²⁴ lid^{JmjC.mut.Scer\UAS.P\T} lid^k06801 lid^{Scer\UAS.P\T.cSa} mor¹ osa² Psc^1.d20 Scer\GAL4^ap-md544 Scer\GAL4^GMR.PF sktl^Δ5 sktl^Δ20 Snr1⁰¹³¹⁹ Su(z)2^1.b7 Trl^s2325 trx^E2 w^+mC
Constructs (6)
	P{GAL4-ninaE.GMR} P{UAS-Cdk4.M} P{UAS-CycD.D} P{UAS-dm.Z} P{UASp-lid.JmjC.mut} P{UASp-lid.S}
Genes (37)
	ago ash1 ash2 Asx Bap60 brm Cdk4 CycD CycE dap dm E2f E(Pc) elav Fib Gcn5 His3 His4 kis lid mor Nop56 Nop60B osa Psc RpI1 RpI135 RpIII128 RpS6 Scer\GAL4 sktl Snr1 Su(z)2 Trl trr trx w
Insertions (1)
	P{GawB}ap^md544
Natural transposons (1)
	P-element