Reference Report

Reference
Citation	Viquez, N.M., Li, C.R., Wairkar, Y.P., DiAntonio, A. (2006). The B' protein phosphatase 2A regulatory subunit well-rounded regulates synaptic growth and cytoskeletal stability at the Drosophila neuromuscular junction. J. Neurosci. 26(36): 9293--9303. (Export to RIS)
FlyBase ID	FBrf0193166
Publication Type	Research paper
PubMed ID	16957085
PubMed Abstract	Synaptic growth is essential for the development and plasticity of neural circuits. To identify molecular mechanisms regulating synaptic growth, we performed a gain-of-function screen for synapse morphology mutants at the Drosophila neuromuscular junction (NMJ). We isolated a B' regulatory subunit of protein phosphatase 2A (PP2A) that we have named well-rounded (wrd). Neuronal overexpression of wrd leads to overgrowth of the synaptic terminal. Endogenous Wrd protein is present in the larval nervous system and muscle and is enriched at central and neuromuscular synapses. wrd is required for normal synaptic development; in its absence, there are fewer synaptic boutons and there is a decrease in synaptic strength. wrd functions presynaptically to promote normal synaptic growth and postsynaptically to maintain normal levels of evoked transmitter release. In the absence of wrd, the presynaptic cytoskeleton is abnormal, with an increased proportion of unbundled microtubules. Reducing PP2A enzymatic activity also leads to an increase in unbundled microtubules, an effect enhanced by reducing wrd levels. Hence, wrd promotes the function of PP2A and is required for normal cytoskeletal organization, synaptic growth, and synaptic function at the Drosophila NMJ.
DOI	10.1523/JNEUROSCI.1740-06.2006
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Neurosci.
Title	Journal of Neuroscience
Publication Year	1981-
ISBN/ISSN	0270-6474 1529-2401
Data from Reference
Aberrations (2)
	Df(3R)189 Df(3R)DG4
Alleles (13)
	futsch^K68 mts^{dn181.Scer\UAS} mts^Scer\UAS.cHa PP2A-B'¹⁰⁴ PP2A-B'^GS628 PP2A-B'^GS676 PP2A-B'^GS2404 PP2A-B'^GS2535 PP2A-B'^RD.Scer\UAS Scer\GAL4^elav.PLu Scer\GAL4^G7 wdb^dn.Scer\UAS wdb^Scer\UAS.cHa
Constructs (7)
	P{GAL4-elav.L} P{GSV1} P{UAS-mts.dn181} P{UAS-mts.H} P{UAS-PP2A-B'.RD} P{UAS-wdb.dn} P{UAS-wdb.H}
Genes (14)
	brp cdm CG7208 CG7215 dlg1 faf futsch GluRIIC mts PP2A-B' Prx5 Scer\GAL4 VGlut wdb
Insertions (5)
	P{GAL4}G7 P{GSV1}PP2A-B'^GS628 P{GSV1}PP2A-B'^GS676 P{GSV1}PP2A-B'^GS2404 P{GSV1}PP2A-B'^GS2535
Natural transposons (1)
	P-element