Reference Report

Reference
Citation	Emmons, R.B., Duncan, D., Duncan, I. (2007). Regulation of the Drosophila distal antennal determinant spineless. Dev. Biol. 302(2): 412--426. (Export to RIS)
FlyBase ID	FBrf0193220
Publication Type	Research paper
PubMed ID	17084833
PubMed Abstract	The transformation of antenna to leg is a classical model for understanding segmental fate decisions in Drosophila. The spineless (ss) gene encodes a bHLH-PAS transcription factor that plays a key role in specifying the identity of distal antennal segments. In this report, we identify the antennal disc enhancer of ss and then use enhancer-lacZ reporters to work out how ss antennal expression is regulated. The antennal determinants Distal-less (Dll) and homothorax (hth) are key activators of the antennal enhancer. Dll is required continuously and, when present at elevated levels, can activate the enhancer in regions devoid of hth expression. In contrast, homothorax (hth) is required only transiently both for activation of the enhancer and for specification of the aristal portion of the antenna. The antennal enhancer is repressed by cut, which determines its proximal limit of expression, and by ectopic Antennapedia (Antp). Repression by Antp is not mediated by hth, suggesting that ss may be a direct target of Antp. Finally, we show that ss+ is not a purely passive target of its regulators: ss+ partially represses hth in the third antennal segment and lies upstream of Dll in the development of the maxillary palp primordia.
DOI	10.1016/j.ydbio.2006.09.044
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (2)
	Df(3R)ss-D114.4 Dp(2;2)D11
Alleles (39)
	al^ex al^Scer\UAS.cKa Antp^Scer\UAS.cMb Avic\GFP^Scer\UAS.cUa ct^Scer\UAS.cLa ct^unspecified dac¹ dac^Scer\UAS.cSa Dll¹⁷ Dll^Scer\UAS.cGa Ecol\lacZ^ss.522 Ecol\lacZ^ss.531 Ecol\lacZ^ss.542 Ecol\lacZ^ss.554 Ecol\lacZ^ss.B6.9 Ecol\lacZ^ss.E1.2 Ecol\lacZ^ss.E1.6 Ecol\lacZ^ss.E1.7 Ecol\lacZ^ss.E1.9 Ecol\lacZ^ss.E2.0 Ecol\lacZ^ss.E5.0 Ecol\lacZ^ss.E6.2 Ecol\lacZ^ss.EX1.9 Ecol\lacZ^ss.EX4.3 Ecol\lacZ^ss.EX6.4 Ecol\lacZ^ss.EX6.5 Ecol\lacZ^ss.EX8.2 Ecol\lacZ^ss.P732 Ecol\lacZ^ss.S4.9 Ecol\lacZ^ss.X8.2 hth^64-1 hth^Meis1-P2 hth^Scer\UAS.cPa hth^unspecified Scer\GAL4^Act5C.PU ss^D114.7 ss^D115.7 ss^Scer\UAS.A5 ss^unspecified
Constructs (28)
	P{ss-lacZ.522} P{ss-lacZ.531} P{ss-lacZ.542} P{ss-lacZ.554} P{ss-lacZ.B6.9} P{ss-lacZ.E1.2} P{ss-lacZ.E1.6} P{ss-lacZ.E1.7} P{ss-lacZ.E1.9} P{ss-lacZ.E2.0} P{ss-lacZ.E5.0} P{ss-lacZ.E6.2} P{ss-lacZ.EX1.9} P{ss-lacZ.EX4.3} P{ss-lacZ.EX6.4} P{ss-lacZ.EX6.5} P{ss-lacZ.EX8.2} P{ss-lacZ.P732} P{ss-lacZ.S4.9} P{ss-lacZ.X8.2} P{UAS-al.K} P{UAS-Antp.Mb} P{UAS-ct.L} P{UAS-dac.S} P{UAS-Dll.G} P{UAS-GFP.U} P{UAS-hth.P} P{UAS-ss.A5}
Genes (11)
	al Antp Avic\GFP ct dac Dll Ecol\lacZ en hth Scer\GAL4 ss
Natural transposons (1)
	P-element
Sequence features (17)
	E1.6 E2.0_522 ss_B6.9 ss_E1.2 ss_E1.7 ss_E1.9 ss_E2.0 ss_E2.0_531 ss_E5.0 ss_E5.0-1 ss_EX6.5 ss_EX8.2 ss_P732 ss_P1190 ss_RP4268 ss_S4.9 ss_X8.2
Transcripts (1)
	Ecol\lacZ^ss.B6.9RA