Reference Report

Reference
Citation	Basto, R., Lau, J., Vinogradova, T., Gardiol, A., Woods, C.G., Khodjakov, A., Raff, J.W. (2006). Flies without centrioles. Cell 125(4): 1375--1386. (Export to RIS)
FlyBase ID	FBrf0193251
Publication Type	Research paper
PubMed ID	16814722
PubMed Abstract	Centrioles and centrosomes have an important role in animal cell organization, but it is uncertain to what extent they are essential for animal development. The Drosophila protein DSas-4 is related to the human microcephaly protein CenpJ and the C. elegans centriolar protein Sas-4. We show that DSas-4 is essential for centriole replication in flies. DSas-4 mutants start to lose centrioles during embryonic development, and, by third-instar larval stages, no centrioles or centrosomes are detectable. Mitotic spindle assembly is slow in mutant cells, and approximately 30% of the asymmetric divisions of larval neuroblasts are abnormal. Nevertheless, mutant flies develop with near normal timing into morphologically normal adults. These flies, however, have no cilia or flagella and die shortly after birth because their sensory neurons lack cilia. Thus, centrioles are essential for the formation of centrosomes, cilia, and flagella, but, remarkably, they are not essential for most aspects of Drosophila development.
DOI	10.1016/j.cell.2006.05.025
Related Publication(s)
Review	Life without centrioles: Cilia in the spotlight. Badano and Katsanis, 2006, Cell 125(7): 1228--1230 [FBrf0192409]
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Cell
Title	Cell
Publication Year	1974-
ISBN/ISSN	0092-8674
Data from Reference
Alleles (8)
	cp309^{PACT.Ubi-p63E.T:Avic\GFP} Ecol\lacZ^Sas-4-s2214 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} rap^{Ubi-p63E.T:Avic\GFP-m6} Sas-4^s2214 Scer\GAL4^elav-C155 Scer\GAL4^insc-Mz1407 αTub84B^{Scer\UAS.P\T.T:Avic\GFP-S65C}
Constructs (4)
	P{UAS-mCD8::GFP.L} P{UASp-GFPS65C-αTub84B} P{Ubi-p63E-GFP-cp309.PACT} P{Ubi-p63E-rap.GFP}
Genes (16)
	aur cnn Cp190 cp309 Ecol\lacZ insc Map60 mira Mmus\Cd8a msps rap Sas-4 Scer\GAL4 tacc αTub84B γTub23C
Insertions (3)
	P{GawB}elav^C155 P{GawB}insc^Mz1407 P{lacW}Sas-4^s2214