Reference Report

Reference
Citation	Hagedorn, E.J., Bayraktar, J.L., Kandachar, V.R., Bai, T., Englert, D.M., Chang, H.C. (2006). Drosophila melanogaster auxilin regulates the internalization of Delta to control activity of the Notch signaling pathway. J. Cell Biol. 173(3): 443--452. (Export to RIS)
FlyBase ID	FBrf0193261
Publication Type	Research paper
PubMed ID	16682530
PubMed Abstract	We have isolated mutations in the Drosophila melanogaster homologue of auxilin, a J-domain-containing protein known to cooperate with Hsc70 in the disassembly of clathrin coats from clathrin-coated vesicles in vitro. Consistent with this biochemical role, animals with reduced auxilin function exhibit genetic interactions with Hsc70 and clathrin. Interestingly, the auxilin mutations interact specifically with Notch and disrupt several Notch-mediated processes. Genetic evidence places auxilin function in the signal-sending cells, upstream of Notch receptor activation, suggesting that the relevant cargo for this auxilin-mediated endocytosis is the Notch ligand Delta. Indeed, the localization of Delta protein is disrupted in auxilin mutant tissues. Thus, our data suggest that auxilin is an integral component of the Notch signaling pathway, participating in the ubiquitin-dependent endocytosis of Delta. Furthermore, the fact that auxilin is required for Notch signaling suggests that ligand endocytosis in the signal-sending cells needs to proceed past coat disassembly to activate Notch.
DOI	10.1083/jcb.200602054
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (2)
	Df(3R)ED5021 Df(3R)ED5092
Alleles (18)
	aux^dsRNA.cHa aux^I670K aux^Scer\UAS.cHa aux^{Scer\UAS.T:Disc\RFP-mRFP} aux^W328X aux^W1150X Clc^{Scer\UAS.T:Avic\GFP-EGFP} Dl^{Scer\UAS.C.T:Disc\RFP-mRFP} Egfr^Scer\UAS.cBa Grasp65^{Scer\UAS.T:Avic\GFP-EGFP} Hsc70-4^{K71S.Scer\UAS} N^264-39 N^ICN.Scer\UAS N^Scer\UAS.cMa Rab11^{Scer\UAS.N.T:Avic\GFP} Scer\GAL4^Act5C.PI Scer\GAL4^GMR.PF T:Disc\RFP^mRFP
Constructs (12)
	P{Act5C-GAL4} P{GAL4-ninaE.GMR} P{UAS-aux.H} P{UAS-aux-RFP} P{UAS-Dl-mRFP} P{UAS-EGFP-Clc} P{UAS-Egfr.B} P{UAS-GFP-Rab11} P{UAS-Grasp65-GFP} P{UAS-Hsc70-4.K71S} P{UAS-N.ICN} P{UAS-N^full}
Genes (12)
	aux boss Clc Dl Egfr elav Grasp65 Hsc70-4 N Rab11 Scer\GAL4 T:Avic\GFP
Natural transposons (1)
	P-element