Reference Report

Reference
Citation	Shigenobu, S., Kitadate, Y., Noda, C., Kobayashi, S. (2006). Molecular characterization of embryonic gonads by gene expression profiling in Drosophila melanogaster. Proc. Natl. Acad. Sci. U.S.A. 103(37): 13728--13733. (Export to RIS)
FlyBase ID	FBrf0193273
Publication Type	Research paper
PubMed ID	16950879
PubMed Abstract	In many animal species, germ-line progenitors associate with gonadal somatic cells to form the embryonic gonads (EGs) that later develop into functional organ producing gametes. To explore the genetic regulation of the germ-line development, we initiated a comprehensive identification and functional analysis of the genes expressed within the EGs. First, we generated a cDNA library from gonads purified from Drosophila embryos by FACS. Using this library, we catalogued the genes expressed in the gonad by EST analysis. A total of 17,218 high-quality ESTs representing 3,051 genes were obtained, corresponding to 20% of the predicted genes in the genome. The EG transcriptome is unexpectedly distinct from that of adult gonads and includes an extremely high proportion of retrotransposon-derived transcripts. We verified 101 genes preferentially expressed in the EGs by whole-mount in situ hybridization. Within this subset, 39 and 58 genes were expressed predominantly in germ-line and somatic cells, respectively, whereas four genes were expressed in the both cell lineages. The gonad-enriched genes encompassed a variety of predicted functions. However, genes implicated in SUMOylation and protein translation, including germ-line-specific ribosomal proteins, are preferentially expressed in the germ line, whereas the expression of various retrotransposons and RNAi-related genes are more prominent in the gonadal soma. These transcriptome data are a resource for understanding the mechanism of various cellular events during germ-line development.
DOI	10.1073/pnas.0603767103
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (1)
	vas^{T:Ivir\HA1,T:Avic\GFP-EGFP}
Constructs (1)
	P{vas.EGFP.HA}
Genes (95)
	14-3-3ε Aos1 armi asl B52 Cam CG1599 CG3036 CG4415 CG5541 CG5800 CG7194 CG7498 CG8908 CG9634 CG9935 CG10565 CG11050 CG11329 CG11447 CG11537 CG11714 CG12576 CG14072 CG14346 CG15784 CG15930 CG17159 CG17162 CG18213 CG44325 CSN3 Dcr-1 Dcr-2 Dd Df31 dpr17 dUTPase ens esg Fas1 FGC026A04 FGM222E05 Fs(2)Ket ftz-f1 Fuca grp Hsp26 Hsp27 Hsp83 janA Kaz-m1 l(2)03709 lbm lwr Mal-A5 Mapmodulin Mcm6 metro Mocs1 mRpS24 mud neur ovo Pdcd4 piwi Ran rgn RpL22 RpL22-like RpS5a RpS5b RpS13 RpS19a RpS19b Rpt6 Sam-S scra Sirup smt3 Ssb-c31a stai stc stg Su(var)205 Swim TfIIA-S Thd1 Top2 Uba1 Uba2 vas Wnt6 zfh1 zpg
Libraries/Collections (1)
	EG_cDNA
Natural transposons (11)
	17.6 297 412 gtwin mdg1 Quasimodo Stalker Stalker2 Tabor Tirant ZAM