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Reference Report
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| Citation | Zielke, N., Querings, S., Grosskortenhaus, R., Reis, T., Sprenger, F. (2006). Molecular dissection of the APC/C inhibitor Rca1 shows a novel F-box-dependent function. EMBO Rep. 7(12): 1266--1272. (Export to RIS) | ||
| FlyBase ID | FBrf0193300 | ||
| Publication Type | Research paper | ||
| PubMed ID | 17099689 | ||
| PubMed Abstract | Rca1 (regulator of Cyclin A)/Emi (early mitotic inhibitor) proteins are essential inhibitors of the anaphase-promoting complex/cyclosome (APC/C). In Drosophila, Rca1 is required during G2 to prevent premature cyclin degradation by the Fizzy-related (Fzr)-dependent APC/C activity. Here, we present a structure and function analysis of Rca1 showing that a carboxy-terminal fragment is sufficient for APC/C inhibition. Rca1/Emi proteins contain a conserved F-box and interact with components of the Skp-Cullin-F-box (SCF) complex. So far, no function has been ascribed to this domain. We find that the F-box of Rca1 is dispensable for APC/C-Fzr inhibition during G2. Nevertheless, we show that Rca1 has an additional function at the G1-S transition, which requires the F-box. Overexpression of Rca1 accelerates the G1-S transition in an F-box-dependent manner. Conversely, S-phase entry is delayed in cells in which endogenous Rca1 is replaced by a transgene lacking the F-box. We propose that Rca1 acts as an F-box protein in an as yet uncharacterized SCF complex, which promotes S-phase entry. | ||
| DOI | 10.1038/sj.embor.7400851 | ||
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| Language of Publication | English | ||
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| Publication Type | Journal | ||
| Abbreviation | EMBO Rep. | ||
| Title | EMBO Reports | ||
| Publication Year | 2000- | ||
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Data from Reference
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| Alleles (13)
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| Constructs (12)
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| Genes (9)
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