Reference Report

Reference
Citation	Tanentzapf, G., Brown, N.H. (2006). An interaction between integrin and the talin FERM domain mediates integrin activation but not linkage to the cytoskeleton. Nat. Cell Biol. 8(6): 601--606. (Export to RIS)
FlyBase ID	FBrf0193338
Publication Type	Research paper
PubMed ID	16648844
PubMed Abstract	Transmembrane adhesion receptors, such as integrins, mediate cell adhesion by interacting with intracellular proteins that connect to the cytoskeleton. Talin, one such linker protein, is thought to have two roles: mediating inside-out activation of integrins, and connecting extracellular matrix (ECM)-bound integrins to the cytoskeleton. Talin's amino-terminal head, which consists of a FERM domain, binds an NPxY motif within the cytoplasmic tail of most integrin beta subunits. This is consistent with the role of FERM domains in recruiting other proteins to the plasma membrane. We tested the role of the talin-head-NPxY interaction in integrin function in Drosophila. We found that introduction of a mutation that perturbs this binding in vitro into the isolated talin head disrupts its recruitment by integrins in vivo. Surprisingly, when engineered into the full-length talin, this mutation did not disrupt talin recruitment by integrins nor its ability to connect integrins to the cytoskeleton. However, it reduced the ability of talin to strengthen integrin adhesion to the ECM, indicating that the function of the talin-head-NPxY interaction is solely to regulate integrin adhesion.
DOI	10.1038/ncb1411
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nat. Cell Biol.
Title	Nature Cell Biology
Publication Year	1999-
ISBN/ISSN	1465-7392 1476-4679
Data from Reference
Aberrations (1)
	Df(3L)79a
Alleles (8)
	if^{ΔGFFNR.Scer\UAS} rhea^{H.Scer\UAS.T:Avic\GFP-m6} rhea^{IBS2.Scer\UAS.T:Avic\GFP} rhea^{R367A.Scer\UAS.IBS1.T:Avic\GFP} rhea^{R367A.Ubi-p63E} rhea^{Scer\UAS.IBS1.T:Avic\GFP} rhea^Ubi-p63E.PT Scer\GAL4^how-24B
Constructs (7)
	P{UAS-if.ΔGFFNR} P{UAS-rhea.IBS1-GFP.R367A} P{UAS-rhea.IBS1-GFP} P{UAS-rhea.IBS2.GFP} P{UAS-talin.H.mGFP} P{Ubi-p63E-talin.R367A} P{Ubi-p63E-talin}
Genes (9)
	Fak if mys Pax rhea Scer\GAL4 stck T:Avic\GFP Tig
Insertions (1)
	P{GawB}how^24B
Natural transposons (1)
	P-element