Reference Report

Reference
Citation	Qi, D., Jin, H., Lilja, T., Mannervik, M. (2006). Drosophila Reptin and other TIP60 complex components promote generation of silent chromatin. Genetics 174(1): 241--251. (Export to RIS)
FlyBase ID	FBrf0193464
Publication Type	Research paper
PubMed ID	16816423
PubMed Abstract	Histone acetyltransferase (HAT) complexes have been linked to activation of transcription. Reptin is a subunit of different chromatin-remodeling complexes, including the TIP60 HAT complex. In Drosophila, Reptin also copurifies with the Polycomb group (PcG) complex PRC1, which maintains genes in a transcriptionally silent state. We demonstrate genetic interactions between reptin mutant flies and PcG mutants, resulting in misexpression of the homeotic gene Scr. Genetic interactions are not restricted to PRC1 components, but are also observed with another PcG gene. In reptin homozygous mutant cells, a Polycomb response-element-linked reporter gene is derepressed, whereas endogenous homeotic gene expression is not. Furthermore, reptin mutants suppress position-effect variegation (PEV), a phenomenon resulting from spreading of heterochromatin. These features are shared with three other components of TIP60 complexes, namely Enhancer of Polycomb, Domino, and dMRG15. We conclude that Drosophila Reptin participates in epigenetic processes leading to a repressive chromatin state as part of the fly TIP60 HAT complex rather than through the PRC1 complex. This shows that the TIP60 complex can promote the generation of silent chromatin.
DOI	10.1534/genetics.106.059980
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (4)
	Df(3L)Pc Df(3R)ea In(1)w^m4h T(1;4)w^m258-21
Alleles (24)
	Ecol\lacZ^{Ubx.PBX.PRE1.6.IDE} esc¹ esc²¹ MRG15^ex1 MRG15^j6A3 Pc¹¹ Pcl⁷ Pcl¹¹ ph-p⁴¹⁰ Psc¹ Psc^k07834 Psc^vgD rept⁰⁶⁹⁴⁵ rept^{Act5C.T:SV5\V5} rept^ex1 rept^{Scer\UAS.P\T.cQa} Scer\GAL4^Act5C.PU Su(var)3-9¹ Su(z)12⁴ Tip60^{MtnA.T:Zzzz\FLAG} w^+mC w^Fab-7.5F24 w^{Mcp.scs'.12:15} w^Mcp.scs'.19
Constructs (5)
	P{Mcp-w#12} P{Mcp-w#19} P{UAS-lacZ.Abd-B.5F24} P{UASp-rept.Q} P{Ubx-lacZ.PBX.PRE1.6.IDE}
Genes (17)
	Ecol\lacZ esc MRG15 Pc Pcl ph-p Psc rept Scer\GAL4 Scr Su(var)3-9 Su(z)12 T:SV5\V5 T:Zzzz\FLAG Tip60 Ubx w
Insertions (9)
	P{hsp26-pt-T}39C-4 P{hsp26-pt-T}39C-5 P{hsp26-pt-T}39C-12 P{hsp26-pt-T}39C-27 P{hsp26-pt-T}39C-72 P{hsp26-pt-T}118E-10 P{hsp26-pt-T}118E-12 P{lacW}MRG15^j6A3 P{PZ}rept⁰⁶⁹⁴⁵
Natural transposons (1)
	P-element