Reference Report

Reference
Citation	Murakami, S., Umetsu, D., Maeyama, Y., Sato, M., Yoshida, S., Tabata, T. (2007). Focal adhesion kinase controls morphogenesis of the Drosophila optic stalk. Development 134(8): 1539--1548. (Export to RIS)
FlyBase ID	FBrf0193558
Publication Type	Research paper
PubMed ID	17360775
PubMed Abstract	Photoreceptor cell axons (R axons) innervate optic ganglia in the Drosophila brain through the tubular optic stalk. This structure consists of surface glia (SG) and forms independently of R axon projection. In a screen for genes involved in optic stalk formation, we identified Fak56D encoding a Drosophila homolog of mammalian focal adhesion kinase (FAK). FAK is a main component of the focal adhesion signaling that regulates various cellular events, including cell migration and morphology. We show that Fak56D mutation causes severe disruption of the optic stalk structure. These phenotypes were completely rescued by Fak56D transgene expression in the SG cells but not in photoreceptor cells. Moreover, Fak56D genetically interacts with myospheroid, which encodes an integrin beta subunit. In addition, we found that CdGAPr is also required for optic stalk formation and genetically interacts with Fak56D. CdGAPr encodes a GTPase-activating domain that is homologous to that of mammalian CdGAP, which functions in focal adhesion signaling. Hence the optic stalk is a simple monolayered structure that can serve as an ideal system for studying glial cell morphogenesis and the developmental role(s) of focal adhesion signaling.
DOI	10.1242/dev.001529
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (2)
	Df(2L)ED1303 Df(2R)BSC26
Alleles (20)
	Avic\GFP^Scer\UAS.cUa CdGAPr^{dsRNA.Scer\UAS} CdGAPr^EY13451 CdGAPr^NP3053 Ecol\lacZ^bi-Pol-1 Ecol\lacZ^ptc-H84 Fak^CG1 Fak^{Scer\UAS.T:Ivir\HA1} Fak^unspecified Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} mys¹ mys¹¹ Scer\GAL4^Act5C.PP Scer\GAL4^{CdGAPr-NP3053} Scer\GAL4^GMR.PY Scer\GAL4^NP2109 Scer\GAL4^NP4702 Scer\GAL4^ptc-559.1 Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} so¹
Constructs (6)
	P{GAL4-Act5C(FRT.CD2).P} P{GMR-GAL4.Y} P{UAS-CdGAPr-dsRNA} P{UAS-Fak.HA} P{UAS-GFP.U} P{UAS-mCD8::GFP.L}
Genes (16)
	Avic\GFP bi CdGAPr chp Ecol\lacZ Fak His3 LanA Mmus\Cd8a mys repo Scer\GAL4 so trol αTub84B αTub84D
Insertions (7)
	P{A92}ptc^H84 P{EPgy2}CdGAPr^EY13451 P{GawB}CdGAPr^NP3053 P{GawB}CG32479^NP4702 P{GawB}NP2109 P{GawB}ptc^559.1 P{lacW}bi^Pol-1