Reference Report

Reference
Citation	Luan, H., Peabody, N.C., Vinson, C.R., White, B.H. (2006). Refined spatial manipulation of neuronal function by combinatorial restriction of transgene expression. Neuron 52(3): 425--436.
FlyBase ID	FBrf0193617
Type of publication	Research paper
Offprint Available	No
External Crossreferences
PubMed ID	17088209
PubMed Abstract	Selective genetic manipulation of neuronal function in vivo requires techniques for targeting gene expression to specific cells. Existing systems accomplish this using the promoters of endogenous genes to drive expression of transgenes directly in cells of interest or, in "binary" systems, to drive expression of a transcription factor or recombinase that subsequently activates the expression of other transgenes. All such techniques are constrained by the limited specificity of the available promoters. We introduce here a combinatorial system in which the DNA-binding (DBD) and transcription-activation (AD) domains of a transcription factor are independently targeted using two different promoters. The domains heterodimerize to become transcriptionally competent and thus drive transgene expression only at the intersection of the expression patterns of the two promoters. We use this system to dissect a neuronal network in Drosophila by selectively targeting expression of the cell death gene reaper to subsets of neurons within the network.
Biosis	2007.32127
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Supplementary material	[FBrf0200193]
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Language of publication	English
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Abbreviation	Neuron
Title	Neuron
Authors
Volume range	1-
Year range	1988-
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Publisher
Place of publication	Cambridge, MA
Language of publication	English
ISBN/ISSN	0896-6273
CODEN	NERNET
Data from Reference
Alleles (21)
	Avic\GFP^{2x.EGFP.Scer\UAS} Ecol\lacZ^Scer\UAS Hsim\VP16^{AD.Ccap.T:Zzzz\ZipEERRL,T:SV40\nls2} Hsim\VP16^{AD.T:Zzzz\ZipEERRL,T:SV40\nls2} Hsim\VP16^{AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2} Hsim\VP16^{ET.AD.T:Zzzz\ZipEERRL,T:SV40\nls2} Hsim\VP16^N4 Hsim\VP16^N6 Scer\GAL4^{AD.Ccap.T:Zzzz\ZipEERRL,T:SV40\nls2} Scer\GAL4^{AD.T:Zzzz\ZipEERRL,T:SV40\nls2} Scer\GAL4^{AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2} Scer\GAL4^Ccap.PP Scer\GAL4^Cha.7.4 Scer\GAL4^{DBD.Act5C.T:Zzzz\ZipRREEL} Scer\GAL4^{DBD.Ccap.T:Zzzz\ZipRREEL} Scer\GAL4^{DBD.Cha.T:Zzzz\ZipRREEL} Scer\GAL4^{DBD.elav.T:Zzzz\ZipRREEL} Scer\GAL4^{ET.AD.T:Zzzz\ZipEERRL,T:SV40\nls2} Scer\GAL4^elav.PLu Scer\GAL80^Ccap.PL rpr^Scer\UAS.C
Constructs (10)
	P{Ccap-GAL4.P} P{Ccap-GAL80.L} P{Cha-GAL4.7.4} P{ET.GAL4.AD} P{ET.VP16.AD} P{GAL4-elav.L} P{UAS-2xEGFP} P{UAS-lacZ.U} P{UAS-rpr.C} P{elav-GAL4.DBD}
Genes (10)
	Avic\GFP Ccap Ecol\lacZ Hsim\VP16 Scer\GAL4 Scer\GAL80 T:SV40\nls2 T:Zzzz\ZipEERRL T:Zzzz\ZipRREEL rpr
Insertions (3)
	P{ET.VP16.AD}N4 P{ET.VP16.AD}N6 P{elav-GAL4.DBD}H9