Reference Report

Reference
Citation	Meulener, M.C., Xu, K., Thomson, L., Ischiropoulos, H., Bonini, N.M. (2006). Mutational analysis of DJ-1 in Drosophila implicates functional inactivation by oxidative damage and aging. Proc. Natl. Acad. Sci. U.S.A. 103(33): 12517--12522. (Export to RIS)
FlyBase ID	FBrf0193931
Publication Type	Research paper
PubMed ID	16894167
PubMed Abstract	Inherited mutations in PARK7, the gene encoding DJ-1, are associated with loss of protein function and early-onset parkinsonism. Like human DJ-1 (hDJ-1), Drosophila DJ-1b protects against oxidative insult and is modified with oxidation. We demonstrate that hDJ-1 rescues flies mutant for DJ-1b, and that a conserved cysteine residue in the fly protein (C104, analogous to C106 in hDJ-1) is critical for biological antioxidant function in vivo. Targeted mutagenesis suggests that modification of DJ-1b at this residue inactivates the protective activity of the protein against oxidative stress. Further studies show that DJ-1 modification increases dramatically with age in flies, mice, and humans, with aged flies showing strikingly increased susceptibility to oxidative stress and markedly enhanced DJ-1b modification upon oxidative challenge. Overoxidation of DJ-1 with age and exposure to oxidative toxins may lead to inactivation of DJ-1 function, suggesting a role in susceptibility to sporadic Parkinson's disease.
DOI	10.1073/pnas.0601891103
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (11)
	dj-1β^{C45A.C104A.Scer\UAS} dj-1β^{C45A.Scer\UAS} dj-1β^{C45D.Scer\UAS} dj-1β^{C104A.Scer\UAS} dj-1β^{C104D.Scer\UAS} dj-1β^Scer\UAS.cMa dj-1β^Δ93 Hsap\PARK7^Scer\UAS.cMa Scer\GAL4^da.G32 Sod^unspecified w^+mC
Constructs (8)
	P{GAL4-da.G32} P{UAS-dj-1β.C45A.C104A} P{UAS-dj-1β.C45A} P{UAS-dj-1β.C45D} P{UAS-dj-1β.C104A} P{UAS-dj-1β.C104D} P{UAS-dj-1β.M} P{UAS-PARK7.M}
Genes (5)
	dj-1β Hsap\PARK7 Scer\GAL4 Sod w
Natural transposons (1)
	P-element