Reference Report

Reference
Citation	Erickson, J.N., Spana, E.P. (2006). Mapping Drosophila genomic aberration breakpoints with comparative genome hybridization on microarrays. Methods Enzymol. 410(): 377--386. (Export to RIS)
FlyBase ID	FBrf0193934
Publication Type	Research paper
PubMed ID	16938561
PubMed Abstract	Chromosomal aberrations are genetic "reagents" that are commonly used in Drosophila research. Stocks containing chromosomes carrying large deletions of DNA (deficiency stocks, designated Df) as well as stocks carrying an extra copy of a chromosomal region (duplication stocks, designated Dp) are essential for a variety of genetic analyses. The extent of what is deleted or duplicated has typically been determined cytologically by salivary gland polytene chromosome squashes, which identify the edges of the aberration (so-called breakpoints) of each Df or Dp at low resolution. The margin of error for this technique can be quite high, however, because it is dependent on the quality of the squash and the experience of the scientist interpreting the data. Comparative genome hybridization on microarrays provides a precise molecular method to identify which regions of the genome are deleted or duplicated in these stocks by examining a change in chromosomal ploidy across the whole genome. Furthermore, this technique allows genetic data obtained with these strains to be placed in a molecular genomic context.
DOI	10.1016/S0076-6879(06)10018-X
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Language of Publication	English
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Publication Type	Compendium
Abbreviation	Methods Enzymol.
Title	Methods in Enzymology
Publication Year	1955-
ISBN/ISSN	0076-6879
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