Reference Report

Reference
Citation	Mandal, L., Martinez-Agosto, J.A., Evans, C.J., Hartenstein, V., Banerjee, U. (2007). A Hedgehog- and Antennapedia-dependent niche maintains Drosophila haematopoietic precursors. Nature 446(7133): 320--324. (Export to RIS)
FlyBase ID	FBrf0194088
Publication Type	Research paper
PubMed ID	17361183
PubMed Abstract	The Drosophila melanogaster lymph gland is a haematopoietic organ in which pluripotent blood cell progenitors proliferate and mature into differentiated haemocytes. Previous work has defined three domains, the medullary zone, the cortical zone and the posterior signalling centre (PSC), within the developing third-instar lymph gland. The medullary zone is populated by a core of undifferentiated, slowly cycling progenitor cells, whereas mature haemocytes comprising plasmatocytes, crystal cells and lamellocytes are peripherally located in the cortical zone. The PSC comprises a third region that was first defined as a small group of cells expressing the Notch ligand Serrate. Here we show that the PSC is specified early in the embryo by the homeotic gene Antennapedia (Antp) and expresses the signalling molecule Hedgehog. In the absence of the PSC or the Hedgehog signal, the precursor population of the medullary zone is lost because cells differentiate prematurely. We conclude that the PSC functions as a haematopoietic niche that is essential for the maintenance of blood cell precursors in Drosophila. Identification of this system allows the opportunity for genetic manipulation and direct in vivo imaging of a haematopoietic niche interacting with blood precursors.
DOI	10.1038/nature05585
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nature
Title	Nature
Publication Year	1869-
ISBN/ISSN	0028-0836
Data from Reference
Alleles (16)
	Antp¹⁷ Antp²⁵ Antp^Scer\UAS.cMb Avic\GFP^Scer\UAS.cUa Avic\GFP^ZCL2897 ci^{Ce-2.Scer\UAS.T:Ivir\HA1} Ecol\lacZ^Ser.II-9.5 hh^ts2 hth^64-1 hth^Scer\UAS.cPa kn^col-1 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^Antp-10 Scer\GAL4^dome-PG14 Scer\GAL4^hs.PB Scer\GAL4^twi.PG
Constructs (8)
	P{GAL4-Hsp70.PB} P{GAL4-twi.G} P{Ser-lacZ.II-9.5} P{UAS-Antp.Mb} P{UAS-ci.Ce-2.HA} P{UAS-GFP.U} P{UAS-hth.P} P{UAS-mCD8::GFP.L}
Genes (14)
	Antp Avic\GFP ci dome Ecol\lacZ Fas3 hh hth kn Mmus\Cd8a odd ptc Scer\GAL4 shg
Insertions (3)
	P{GawB}Antp-10 P{GawB}dome^PG14 P{PTT-un1}ZCL2897