Reference Report

Reference
Citation	Joly, W., Mugat, B., Maschat, F. (2007). Engrailed controls the organization of the ventral nerve cord through frazzled regulation. Dev. Biol. 301(2): 542--554. (Export to RIS)
FlyBase ID	FBrf0194180
Publication Type	Research paper
PubMed ID	17126316
PubMed Abstract	In Drosophila, the ventral nerve cord (VNC) architecture is built from neuroblasts that are specified during embryonic development, mainly by transcription factors. Here we show that Engrailed, a homeodomain transcription factor known to be involved in the establishment of neuroblast identity, is also directly implicated in the regulation of axonal guidance cues. Posterior commissures (PC) are missing in engrailed mutant embryos, and axonal pathfinding defects are observed when Engrailed is ectopically expressed at early stages, prior to neuronal specification. We also show that frazzled, enabled, and trio, all of which are potential direct targets of Engrailed and are involved in axonal navigation, interact genetically with engrailed to form posterior commissures in the developing VNC. The regulation of frazzled expression in engrailed-expressing neuroblasts contributes significantly to the formation of the posterior commissures by acting on axon growth. Finally, we identified a small genomic fragment within intron 1 of frazzled that can mediate activation by Engrailed in vivo when fused to a GFP reporter. These results indicate that Engrailed's function during the segregation of the neuroblasts is crucial for regulating different actors that are later involved in axon guidance.
DOI	10.1016/j.ydbio.2006.10.019
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (2)
	Df(2R)en^E Df(2R)en-SFX31
Alleles (28)
	Avic\GFP^{S65T.Scer\UAS} en⁵⁴ en^E en^Scer\UAS.cTa en^{Scer\UAS.Δ282.T:Hsim\VP16} ena⁰²⁰²⁹ ena^GC5 fra¹ fra² fra³ fra⁴ fra^Scer\UAS.cKa inv³⁰ inv^E Mmus\Cd8a^{fra-2C5.hs.T:Avic\GFP} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^Bx-MS1096 Scer\GAL4^da.G32 Scer\GAL4^eg-Mz360 Scer\GAL4^elav.PLu Scer\GAL4^en-e16E Scer\GAL4^insc-Mz1407 Scer\GAL4^Kr.PM Scer\GAL4^prd.RG1 Scer\GAL4^sca.PC trio^6A trio^KG06642 ttk⁸⁰⁴
Constructs (11)
	P{2C5-mCD8-GFP} P{GAL4-da.G32} P{GAL4-elav.L} P{GAL4-Kr.C} P{GAL4-prd.F} P{sca-Gal-4} P{UAS-en.T} P{UAS-en.Δ282::VP16} P{UAS-fra} P{UAS-GFP.S65T} P{UAS-mCD8::GFP.L}
Genes (10)
	Avic\GFP en ena fra inv Mmus\Cd8a Scer\GAL4 T:Avic\GFP trio ttk
Insertions (4)
	P{en2.4-GAL4}e16E P{GawB}Bx^MS1096 P{GawB}eg^Mz360 P{GawB}insc^Mz1407
Natural transposons (1)
	P-element
Sequence features (1)
	fra_2C5