Reference Report

Reference
Citation	Lovegrove, B., Simoes, S., Rivas, M.L., Sotillos, S., Johnson, K., Knust, E., Jacinto, A., Castelli-Gair, H. (2006). Coordinated control of cell adhesion, polarity, and cytoskeleton underlies Hox-induced organogenesis in Drosophila. Curr. Biol. 16(22): 2206--2216. (Export to RIS)
FlyBase ID	FBrf0194228
Publication Type	Research paper
PubMed ID	17113384
PubMed Abstract	Hox genes control animal body plans by directing the morphogenesis of segment-specific structures. As transcription factors, HOX proteins achieve this through the activation of downstream target genes. Much research has been devoted to the search for these targets and the characterization of their roles in organogenesis. This has shown that the direct targets of Hox activation are often transcription factors or signaling molecules, which form hierarchical genetic networks directing the morphogenesis of particular organs. Importantly, very few of the direct Hox targets known are "realizator" genes involved directly in the cellular processes of organogenesis.Here, we describe for the first time a complete network linking the Hox gene Abdominal-B to the realizator genes it controls during the organogenesis of the external respiratory organ of the larva. In this process, Abdominal-B induces the expression of four intermediate signaling molecules and transcription factors, and this expression results in the mosaic activation of several realizator genes. The ABD-B spiracle realizators include at least five cell-adhesion proteins, cell-polarity proteins, and GAP and GEF cytoskeleton regulators. Simultaneous ectopic expression of the Abd-B downstream targets can induce spiracle-like structure formation in the absence of ABD-B protein.Hox realizators include cytoskeletal regulators and molecules required for the apico-basal cell organization. HOX-coordinated activation of these realizators in mosaic patterns confers to the organ primordium its assembling properties. We propose that during animal development, Hox-controlled genetic cascades coordinate the local cell-specific behaviors that result in organogenesis of segment-specific structures.
DOI	10.1016/j.cub.2006.09.029
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Supplementary material	Supplemental Data. [FBrf0220724]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Aberrations (2)
	Df(1)os1A2 Df(2L)32FP-5
Alleles (34)
	Abd-B^M1 Abd-B^Scer\UAS.cCa Cad74A^{Scer\UAS.T:Avic\GFP-EGFP} Cad88C^a.cLa Cad88C^cLa Cad96Cb^a.cLa Cad96Cb^cLa crb^D88-3 crb^{T6AT9AS11AS13A.intra.Scer\UAS} ct^db7 ct^Scer\UAS.cLa cv-c⁷ dome^G0468 Ecol\lacZ^crb.43.2 Ecol\lacZ^crb.STAT.mut Ecol\lacZ^grh.NB ems² ems^Scer\UAS.cSa grn^7J grn^Scer\UAS.cBa os^Scer\UAS.cZa Rac1^L89.Scer\UAS Rac1^V12.Scer\UAS Rho1^N19.Scer\UAS Rho1^V14.Scer\UAS Scer\GAL4^69B Scer\GAL4^ems.HRE Scer\GAL4^h-H10 Scer\GAL4^klu-G410 Scer\GAL4^Kr.PM shg² Stat92E⁰⁶³⁴⁶ T:Avic\GFP^EGFP w^+mC
Constructs (14)
	P{crb-lacZ.43.2} P{ems-GAL4.HRE} P{GAL4-Kr.C} P{grh-lacZ.NB} P{UAS-Cad74A.EGFP} P{UAS-crb.intra.T6AT9AS11AS13A} P{UAS-ct.L} P{UAS-ems.S} P{UAS-grn.B} P{UAS-os.Z} P{UAS-Rac1.L89} P{UAS-Rac1.V12} P{UAS-Rho1.N19} P{UAS-Rho1.V14}
Genes (25)
	Abd-B Cad74A Cad86C Cad88C Cad96Cb crb ct cv-c dlg1 dome Ecol\lacZ ems grh grn Nrt os Rac1 Rho1 RhoGEF64C salm sas Scer\GAL4 shg Stat92E w
Insertions (3)
	P{GAL4}h^H10 P{GAL4}klu^G410 P{GawB}69B
Natural transposons (1)
	P-element
Sequence features (1)
	crb_43.2