Reference Report

Reference
Citation	Malpel, S., Claret, S., Sanial, M., Brigui, A., Piolot, T., Daviet, L., Martin-Lanneree, S., Plessis, A. (2007). The last 59 amino acids of Smoothened cytoplasmic tail directly bind the protein kinase Fused and negatively regulate the Hedgehog pathway. Dev. Biol. 303(1): 121--133. (Export to RIS)
FlyBase ID	FBrf0194316
Publication Type	Research paper
PubMed ID	17182028
PubMed Abstract	The Hedgehog (HH) signaling pathway is crucial for the development of many organisms and its inappropriate activation is involved in numerous cancers. HH signal controls the traffic and activity of the seven-pass transmembrane protein Smoothened (SMO), leading to the transcriptional regulation of HH-responsive genes. In Drosophila, the intracellular transduction events following SMO activation depend on cytoplasmic multimeric complexes that include the Fused (FU) protein kinase. Here we show that the regulatory domain of FU physically interacts with the last 52 amino acids of SMO and that the two proteins colocalize in vivo to vesicles. The deletion of this region of SMO leads to a constitutive activation of SMO, promoting the ectopic transcription of HH target genes. This activation is partially dependent of FU activity. Thus, we identify a novel link between SMO and the cytoplasmic complex(es) and reveal a negative role of the SMO C-terminal region that interacts with FU. We propose that FU could act as a switch, activator in presence of HH signal or inhibitor in absence of HH.
DOI	10.1016/j.ydbio.2006.10.042
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Alleles (18)
	cos^{Act5C.T:Disc\RFP-mRFP} Ecol\lacZ^dpp.BS3.0 fu¹ fu⁵⁴ fu^{Act5C.m6.T:Avic\GFP} fu^{Act5C.T:Disc\RFP-mRFP} fu^RX2 fu^{Scer\UAS.T:Avic\GFP} Scer\GAL4^71B Scer\GAL4^Bx-MS1096 Scer\GAL4^da.G32 smo^{Act5C.T:Avic\GFP-EGFP} smo^D16 smo^{Scer\UAS.J.T:Avic\GFP} smo^{ΔFU.Act5C.T:Avic\GFP-EGFP} smo^{ΔFU.Scer\UAS} T:Avic\GFP^EGFP T:Disc\RFP^mRFP
Constructs (10)
	P{Act5C-m6GFP-fu} P{Act5C-mRFP-cos} P{Act5C-mRFP-fu} P{Act5C-smo.ΔFU-EGFP} P{Act5C-smo-EGFP} P{BS3.0} P{GAL4-da.G32} P{UAS-GFP-fu.M} P{UAS-smo.GFP} P{UAS-smo.ΔFU</up>}
Genes (9)
	ci cos dpp Ecol\lacZ fu ptc Scer\GAL4 smo T:Avic\GFP
Insertions (2)
	P{GawB}71B P{GawB}Bx^MS1096
Natural transposons (1)
	P-element