Reference Report

Reference
Citation	Vagin, V.V., Sigova, A., Li, C., Seitz, H., Gvozdev, V., Zamore, P.D. (2006). A distinct small RNA pathway silences selfish genetic elements in the germline. Science 313(5785): 320--324. (Export to RIS)
FlyBase ID	FBrf0194520
Publication Type	Research paper
PubMed ID	16809489
PubMed Abstract	In the Drosophila germline, repeat-associated small interfering RNAs (rasiRNAs) ensure genomic stability by silencing endogenous selfish genetic elements such as retrotransposons and repetitive sequences. Whereas small interfering RNAs (siRNAs) derive from both the sense and antisense strands of their double-stranded RNA precursors, rasiRNAs arise mainly from the antisense strand. rasiRNA production appears not to require Dicer-1, which makes microRNAs (miRNAs), or Dicer-2, which makes siRNAs, and rasiRNAs lack the 2',3' hydroxy termini characteristic of animal siRNA and miRNA. Unlike siRNAs and miRNAs, rasiRNAs function through the Piwi, rather than the Ago, Argonaute protein subfamily. Our data suggest that rasiRNAs protect the fly germline through a silencing mechanism distinct from both the miRNA and RNA interference pathways.
DOI	10.1126/science.1129333
Related Publication(s)
Supplementary material	Supporting Online Material. [FBrf0198717]
Review	Molecular biology. A new RNA dimension to genome control. Carthew, 2006, Science 313(5785): 305--306 [FBrf0192199] RasiRNAs battle selfish genetic elements. Carmi, 2006, Cell 126(2): 223--224 [FBrf0198709]
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	Science
Title	Science
Publication Year	1895-
ISBN/ISSN	0036-8075
Data from Reference
Aberrations (1)
	Df(YL)Su(Ste)1
Alleles (13)
	AGO2^51B AGO2⁴¹⁴ armi^72.1 aub^HN2 aub^QC42 aub^{Scer\UAS.P\T.T:Avic\GFP-m6} Dcr-2^L811fsX loqs^f00791 piwi² piwi^{hs.T:Hsap\MYC} r2d2¹ spn-E^100.37 w^dsRNA.GMR
Constructs (1)
	P{GMR-w.IR}
Genes (17)
	AGO1 AGO2 AGO3 armi aub ban Dcr-1 Dcr-2 loqs mir-8 mir-311 Mst40 piwi r2d2 spn-E Su(Ste) w
Natural transposons (5)
	gypsy HeT-A I-element mdg1 roo