Reference Report

Reference
Citation	Zhang, Q., Zhang, L., Wang, B., Ou, C.Y., Chien, C.T., Jiang, J. (2006). A hedgehog-induced BTB protein modulates hedgehog signaling by degrading Ci/Gli transcription factor. Dev. Cell 10(6): 719--729. (Export to RIS)
FlyBase ID	FBrf0194555
Publication Type	Research paper
PubMed ID	16740475
PubMed Abstract	The Ci/Gli family of transcription factors mediates Hedgehog (Hh) signaling in many key developmental processes. Here we identify a Hh-induced MATH and BTB domain containing protein (HIB) as a negative regulator of the Hh pathway. Overexpressing HIB down regulates Ci and blocks Hh signaling, whereas inactivating HIB results in Ci accumulation and enhanced pathway activity. HIB binds the N- and C-terminal regions of Ci, both of which mediate Ci degradation. HIB forms a complex with Cul3, a scaffold for modular ubiquitin ligases, and promotes Ci ubiquitination and degradation through Cul3. Furthermore, HIB-mediated Ci degradation is stimulated by Hh and inhibited by Suppressor of Fused (Sufu). The mammalian homolog of HIB, SPOP, can functionally substitute for HIB, and Gli proteins are degraded by HIB/SPOP in Drosophila. We provide evidence that HIB prevents aberrant Hh signaling posterior to the morphogenic furrow, which is essential for normal eye development.
DOI	10.1016/j.devcel.2006.05.004
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
ISBN/ISSN
Data from Reference
Aberrations (1)
	Df(3R)Exel6171
Alleles (28)
	Avic\GFP^Scer\UAS.cUa ci^{-3P.Scer\UAS.W.T:Ivir\HA1} ci^{76.Scer\UAS.T:Ivir\HA1} ci^{Scer\UAS.W.T:Ivir\HA1} ci^{ΔN1.Scer\UAS.T:Ivir\HA1} ci^{ΔN2.Scer\UAS.T:Ivir\HA1} ci^{ΔNC1.Scer\UAS.T:Ivir\HA1} ci^{ΔNC2.Scer\UAS.T:Ivir\HA1} Cul-3^gft2 Cul-3^{Scer\UAS.T:Zzzz\FLAG} Ecol\lacZ^dpp.BS3.0 Ecol\lacZ^ptc.PG Ecol\lacZ^rdx-03477 hh^Scer\UAS.cAa Hsap\GLI3^{Scer\UAS.T:Hsap\MYC} Mmus\Spop^{Scer\UAS.T:Zzzz\FLAG} rdx⁰³⁴⁷⁷ rdx^{dsRNA.Scer\UAS.cZa} rdx^{Scer\UAS.T:Zzzz\FLAG} rdx^Δ6 rdx^Δ14 Scer\GAL4^ap-md544 Scer\GAL4^Bx-MS1096 Scer\GAL4^Eq1 Scer\GAL4^GMR.PF Su(fu)^{Scer\UAS.T:Hsap\MYC} w^+mC Xlae\Gli2^{Scer\UAS.T:Hsap\MYC}
Constructs (19)
	P{BS3.0} P{GAL4-ninaE.GMR} P{ptcA-lacZ} P{UAS-ci.HA.-3P} P{UAS-ci.HA.76} P{UAS-ci.HA.W} P{UAS-ciΔN1} P{UAS-ciΔN2} P{UAS-ciΔNC1} P{UAS-ciΔNC2} P{UAS-Flag-Cul3} P{UAS-Flag-Spop} P{UAS-GFP.U} P{UAS-hh.A} P{UAS-Myc-Hsap\GLI3} P{UAS-mycSu(fu)} P{UAS-Myc-Xlae\Gli2} P{UAS-rdx.Flag} P{UAS-rdx.RNAi.Z}
Genes (16)
	Avic\GFP ci Cul-3 dpp Ecol\lacZ elav hh Hsap\GLI3 Mmus\Spop ptc rdx Scer\GAL4 Su(fu) T:Zzzz\FLAG w Xlae\Gli2
Insertions (4)
	P{GAL4-Hsp70.PB}l(3)Eq1^Eq1 P{GawB}ap^md544 P{GawB}Bx^MS1096 P{PZ}rdx⁰³⁴⁷⁷
Natural transposons (1)
	P-element