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Citation
Kunte, A.S., Matthews, K.A., Rawson, R.B. (2006). Fatty acid auxotrophy in Drosophila larvae lacking SREBP.  Cell Metab. 3(6): 439--448.
FlyBase ID
FBrf0194588
Publication Type
Research paper
Abstract

SREBPs are membrane bound transcription factors that are crucial for normal lipid synthesis in animal cells. Here, we show that Drosophila lacking dSREBP die before the third larval instar. Mutant larvae exhibit pronounced growth defects prior to lethality, along with substantial deficits in the transcription of genes required for fatty acid synthesis. Compared to wild-type larvae, mutants contain markedly less fatty acid, although its composition is unaltered. Dietary supplementation with fatty acids rescues mutants to adulthood. The most effective fatty acid, oleate, rescues 80% of homozygotes. Rescue by dSREBP requires expression only in fat body and gut. Larvae expressing dSREBP prior to pupariation complete development and are viable as adults even when dSREBP expression is subsequently extinguished. The role, if any, of dSREBP in adults is not yet apparent. These data indicate that dSREBP deficiency renders Drosophila larvae auxotrophic for fatty acids.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell Metab.
    Title
    Cell Metabolism
    Publication Year
    2005-
    ISBN/ISSN
    1550-4131
    Data From Reference
    Aberrations (1)
    Alleles (13)
    Genes (11)
    Natural transposons (1)
    Insertions (6)
    Experimental Tools (2)
    Transgenic Constructs (5)
    Transcripts (4)