Reference Report

Reference
Citation	Ninov, N., Chiarelli, D.A., Martin-Blanco, E. (2007). Extrinsic and intrinsic mechanisms directing epithelial cell sheet replacement during Drosophila metamorphosis. Development 134(2): 367--379. (Export to RIS)
FlyBase ID	FBrf0194691
Publication Type	Research paper
PubMed ID	17166923
PubMed Abstract	The fusion of epithelial sheets is an essential morphogenetic event. Here, we study the development of the abdomen of Drosophila as a model of bounded epithelia expansion and uncover a complex multistep process for the generation of the adult epidermis from histoblasts, founder cells that replace the larval cells during metamorphosis. We find that histoblasts experience a biphasic cell cycle and emit apical projections that direct their invasive planar intercalation in between larval cells. Coordinately, the larval cells extrude from the epithelia by apical constriction of an actomyosin ring and as a consequence die by apoptosis and are removed by circulating haemocytes. We demonstrate that the proliferation of histoblasts and the death of larval cells are triggered by two independent extrinsic Ecdysone hormonal pulses. Finally, we show that histoblast spreading and the death of larval cells depend on a mutual exchange of signals and are non-autonomous processes.
DOI	10.1242/dev.02728
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (20)
	Act5C^{Scer\UAS.P\T.T:Avic\GFP} Avic\GFP^{E.Scer\UAS.T:Uuuu\Myr4} Avic\GFP^Scer\UAS.cUa Avic\GFP^{Scer\UAS.m6.T:Btau\MAPT} BacA\p35^Scer\UAS.cHa chic^Scer\UAS.cCa dap^Scer\UAS.cLa EcR^{B1-ΔC655.F645A.Scer\UAS} EcR^{dsRNA.Scer\UAS.cCa} Mbs^{N300.Scer\UAS} Rac1^V12.Scer\UAS rok^CAT.Scer\UAS Scer\GAL4^Act5C.PI Scer\GAL4^esg-NP5130 Scer\GAL4^NP0056 Scer\GAL4^{Scer\FRT.Act5C} Scer\GAL4^srp.Hemo shg^{Ubi-p63E.T:Avic\GFP-rs} sqh^{RLC.T:Avic\GFP-S65T} Vang^{Act5C.T:Avic\GFP-YFP}
Constructs (17)
	P{Act5C-GAL4} P{Act5C-Vang.YFP} P{AyGAL4} P{sqh-GFP.RLC} P{srp.Hemo-GAL4} P{UAS-chic.C} P{UAS-dap.L} P{UAS-EcR.B1-ΔC655.F645A} P{UAS-EcR-RNAi} P{UAS-GFP.U} P{UAS-Mbs.N300} P{UASp-Act5C.T:GFP} P{UAS-Rac1.V12} P{UAS-rok.CAT} P{UAS-srcEGFP} P{UAS-Tau-GFP.m6} P{Ubi-p63E-shg.GFP}
Genes (14)
	Act5C Avic\GFP BacA\p35 chic dap EcR Fas3 Mbs Rac1 rok Scer\GAL4 shg sqh Vang
Insertions (2)
	P{GawB}kibra^NP0056 P{GawB}NP5130