Reference Report

Reference
Citation	Matakatsu, H., Blair, S.S. (2006). Separating the adhesive and signaling functions of the Fat and Dachsous protocadherins. Development 133(12): 2315--2324. (Export to RIS)
FlyBase ID	FBrf0194700
Publication Type	Research paper
PubMed ID	16687445
PubMed Abstract	The protocadherins Fat (Ft) and Dachsous (Ds) are required for several processes in the development of Drosophila, including controlling growth of imaginal discs, planar cell polarity (PCP) and the proximodistal patterning of appendages. Ft and Ds bind in a preferentially heterophilic fashion, and Ds is expressed in distinct patterns along the axes of polarity. It has thus been suggested that Ft and Ds serve not as adhesion molecules, but as receptor and ligand in a poorly understood signaling pathway. To test this hypothesis, we performed a structure-function analysis of Ft and Ds, separating their adhesive and signaling functions. We found that the extracellular domain of Ft is not required for its activity in growth, PCP and proximodistal patterning. Thus, ligand binding is not necessary for Ft activity. By contrast, the extracellular domain of Ds is necessary and sufficient to mediate its effects on PCP, consistent with the model that Ds acts as a ligand during PCP. However, we also provide evidence that Ds can regulate growth independently of Ft, and that the intracellular domain of Ds can affect proximodistal patterning, both suggestive of functions independent of binding Ft. Finally, we show that ft mutants or a dominant-negative Ft construct can affect disc growth without changes in the expression of wingless and Wingless target genes.
DOI	10.1242/dev.02401
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Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (25)
	Avic\GFP^Scer\UAS.cUa ds⁰⁵¹⁴² ds^gal4 ds^Scer\UAS.cTa ds^UAO71 ds^{ΔECD.Scer\UAS} ds^{ΔICD.Scer\UAS} Ecol\lacZ^wg-en11 ft⁸ ft^G-rv ft^k07918 ft^Scer\UAS.cMa ft^{ΔECD.Scer\UAS} ft^{ΔICD.Scer\UAS.T:Ivir\HA1} Scer\GAL4^30A Scer\GAL4^Act5C.PI Scer\GAL4^ap-md544 Scer\GAL4^da.G32 Scer\GAL4^Dll-md23 Scer\GAL4^ds-gal4 Scer\GAL4^en-e16E Scer\GAL4^hh-Gal4 Scer\GAL4^salm-459.2 Scer\GAL4^αTub84B.PL w^+mC
Constructs (11)
	P{Act5C-GAL4} P{GAL4-da.G32} P{GawB} P{tubP-GAL4} P{UAS-ds.T} P{UAS-ds.ΔECD} P{UAS-ds.ΔICD} P{UAS-ft.M} P{UAS-ft.ΔECD} P{UAS-ft.ΔICD.HA} P{UAS-GFP.U}
Genes (11)
	Avic\GFP Dll ds Ecol\lacZ ft Scer\GAL4 shg T:Ivir\HA1 vg w wg
Insertions (8)
	P{en1}wg^en11 P{en2.4-GAL4}e16E P{GAL4}hh^Gal4 P{GawB}30A P{GawB}459.2 P{GawB}ap^md544 P{GawB}Dll^md23 P{GawB}ds^gal4
Natural transposons (1)
	P-element