Reference Report

Reference
Citation	Hennig, K.M., Colombani, J., Neufeld, T.P. (2006). TOR coordinates bulk and targeted endocytosis in the Drosophila melanogaster fat body to regulate cell growth. J. Cell Biol. 173(6): 963--974. (Export to RIS)
FlyBase ID	FBrf0195068
Publication Type	Research paper
PubMed ID	16785324
PubMed Abstract	Target of rapamycin (TOR) is a central regulator of cellular and organismal growth in response to nutrient conditions. In a genetic screen for novel TOR interactors in Drosophila melanogaster, we have identified the clathrin-uncoating ATPase Hsc70-4, which is a key regulator of endocytosis. We present genetic evidence that TOR signaling stimulates bulk endocytic uptake and inhibits the targeted endocytic degradation of the amino acid importer Slimfast. Thus, TOR simultaneously down-regulates aspects of endocytosis that inhibit growth and up-regulates potential growth-promoting functions of endocytosis. In addition, we find that disruption of endocytosis leads to changes in TOR and phosphatidylinositol-3 kinase activity, affecting cell growth, autophagy, and rapamycin sensitivity. Our data indicate that endocytosis acts both as an effector function downstream of TOR and as a physiologically relevant regulator of TOR signaling.
DOI	10.1083/jcb.200511140
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Alleles (35)
	Avic\GFP^Scer\UAS.cUa Clc^{Scer\UAS.T:Avic\GFP-EGFP} Disc\RFP^{Scer\UAS.T:Myr-Src64B} Ecol\lacZ^dpp.BS3.0 gig^Scer\UAS.cTa Hrs^D28 Hsc70-4^54.1 Hsc70-4¹⁹⁵ Hsc70-4^+t14 Hsc70-4^e3 Hsc70-4^e19 Hsc70-4^{K71S.Scer\UAS} Hsc70-4^Wee-P1 Hsc70-4^Δ16 Nedd4^{C974FS.Scer\UAS.T:Ivir\HA1} Rab5^{S43N.Scer\UAS.T:Avic\GFP} Rab5^{Scer\UAS.T:Avic\GFP-EGFP} Rab7^{Scer\UAS.T:Avic\GFP} Rheb^EP50.084 S6k^l-1 Scer\GAL4^Bx-MS1096 Scer\GAL4^Cg.PA Scer\GAL4^en-e16E Scer\GAL4^ey.PH Scer\GAL4^fat Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} shi^{K44A.Scer\UAS} slif¹ slif^Scer\UAS.cCa Thor^Scer\UAS.cMa Tor^{ey.hs.T:Zzzz\FLAG} Tor^{WT.Scer\UAS.T:Zzzz\FLAG} Tor^ΔP Tsc1²⁹ Tsc1^Scer\UAS.cTa
Constructs (19)
	P{BS3.0} P{Cg-GAL4.A} P{ey-TOR.FLAG} P{GAL4-Act5C(FRT.CD2).P} P{GAL4-ey.H} P{Hsc70-4.14} P{UAS-EGFP-Clc} P{UAS-GFP.U} P{UAS-GFP-Rab5} P{UAS-GFP-Rab5S43N} P{UAS-gig.T} P{UAS-Hsc70-4.K71S} P{UAS-myr-mRFP} P{UAS-Rab7.GFP} P{UAS-shi.K44A} P{UAS-slif.C} P{UAS-Thor.wt} P{UAS-Tor.WT} P{UAS-Tsc1.T}
Genes (22)
	Akt1 Avic\GFP Clc Disc\RFP Ecol\lacZ foxo gig Hrs Hsc70-4 Lsp2 Nedd4 Rab5 Rab7 Rheb S6k Scer\GAL4 shi slif T:Zzzz\FLAG Thor Tor Tsc1
Insertions (6)
	P{Cg-GAL4.A}2 P{en2.4-GAL4}e16E P{EP.Scer\UAS(loxP.Scer\UAS.y⁺).BN}Rheb^EP50.084 P{GAL4}fat P{GawB}Bx^MS1096 P{Wee-P.ph0}Hsc70-4^Wee-P1
Natural transposons (1)
	P-element