Reference Report

Reference
Citation	Altenhein, B., Becker, A., Busold, C., Beckmann, B., Hoheisel, J.D., Technau, G.M. (2006). Expression profiling of glial genes during Drosophila embryogenesis. Dev. Biol. 296(2): 545--560. (Export to RIS)
FlyBase ID	FBrf0195367
Publication Type	Research paper
PubMed ID	16762338
PubMed Abstract	In the central nervous system of Drosophila, the induction of the glial cell fate is dependent on the transcription factor glial cells missing (gcm). Though a considerable number of other genes have been shown to be expressed in all or in subsets of glial cells, the course of glial cell differentiation and subtype specification is only poorly understood. This prompted us to design a whole genome microarray approach comparing gcm gain-of-function and, for the first time, gcm loss-of-function genetics to wildtype in time course experiments along embryogenesis. The microarray data were analyzed with special emphasis on the temporal profile of differential regulation. A comparison of both experiments enabled us to identify more than 300 potential gcm target genes. Validation by in situ hybridization revealed expression in glial cells, macrophages, and tendon cells (all three cell types depend on gcm) for 70 genes, of which more than 50 had been unknown to be under gcm control. Eighteen genes are exclusively expressed in glial cells, and their dependence on gcm was confirmed in situ. Initial considerations regarding the role of the newly discovered glial genes are discussed based on gene ontology and the temporal profile and subtype specificity of their expression. This collection of glial genes provides an important basis for the clarification of the genetic network controlling various aspects of glial development and function.
DOI	10.1016/j.ydbio.2006.04.460
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Alleles (6)
	Avic\GFP^{S65T.Scer\UAS} Avic\GFP^{S65T.Ubi-p63E} gcm^N7-4 gcm^Scer\UAS.cJa Scer\GAL4^Kr.PM Scer\GAL4^Mz1060
Constructs (4)
	P{GAL4-Kr.C} P{UAS-gcm.J} P{UAS-GFP.S65T} P{Ubi-GFP.S65T}
Genes (74)
	Aats-phe alrm Argk Avic\GFP Cam Caps Cen CG1677 CG2051 CG2852 CG3168 CG3408 CG4266 CG4936 CG5080 CG6218 CG6418 CG7433 CG7966 CG8066 CG8965 CG9336 CG9338 CG9796 CG10326 CG10591 CG11164 CG11652 CG15141 CG43164 Cip4 CR42862 crol csw Cys dup dUTPase elav fabp Flo-1 Fpps gcm Gdi glu GstE12 insv LanB1 Map60 mgl moody nerfin-1 NimC4 nrv2 NT1 Osi7 pain Pink1 pnut proPO45 Ptp10D Pvr repo RpII140 Scer\GAL4 scf smid Spn spz6 su(r) tap ttk Uba2 UbcD2 zyd
Insertions (1)
	P{GawB}Mz1060