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Citation
Molnar, C., Lopez-Varea, A., Hernandez, R., de Celis, J.F. (2006). A gain-of-function screen identifying genes required for vein formation in the Drosophila melanogaster wing.  Genetics 174(3): 1635--1659.
FlyBase ID
FBrf0195387
Publication Type
Research paper
Abstract
The formation of the Drosophila wing involves developmental processes such as cell proliferation, pattern formation, and cell differentiation that are common to all multicellular organisms. The genes controlling these cellular behaviors are conserved throughout the animal kingdom, and the genetic analysis of wing development has been instrumental in their identification and functional characterization. The wing is a postembryonic structure, and most loss-of-function mutations are lethal in homozygous flies before metamorphosis. In this manner, loss-of-function genetic screens aiming to identify genes affecting wing formation have not been systematically utilized. As an alternative, a number of genetic searches have utilized the phenotypic consequences of gene gain-of-expression, as a method more efficient to search for genes required during imaginal development. Here we present the results of a gain-of-function screen designed to identify genes involved in the formation of the wing veins. We generated 13,000 P-GS insertions of a P element containing UAS sequences (P-GS) and combined them with a Gal4 driver expressed mainly in the developing pupal veins. We selected 500 P-GSs that, in combination with the Gal4 driver, result in modifications of the veins, changes in the morphology of the wing, or defects in the differentiation of the trichomes. The P-element insertion sites were mapped to the genomic sequence, identifying 373 gene candidates to participate in wing morphogenesis and vein formation.
PubMed ID
PubMed Central ID
PMC1667087 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase
Mapping of GS insertion lines from Jose de Celis.
de Celis et al., 2013.9.11, Mapping of GS insertion lines from Jose de Celis. [FBrf0222651]
P{GSV1}C282[C282] and P{GSV1}zf30C[s-244].
de Celis, 2014.12.15, P{GSV1}C282[C282] and P{GSV1}zf30C[s-244]. [FBrf0227028]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference
    Alleles (272)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Genes (608)
    Natural transposons (1)
    Insertions (256)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Experimental Tools (2)
    Transgenic Constructs (25)
    Transcripts (1)