Reference Report

Reference
Citation	Gorczyca, D., Ashley, J., Speese, S., Gherbesi, N., Thomas, U., Gundelfinger, E., Gramates, L.S., Budnik, V. (2007). Postsynaptic membrane addition depends on the Discs-Large-interacting t-SNARE Gtaxin. J. Neurosci. 27(5): 1033--1044. (Export to RIS)
FlyBase ID	FBrf0195390
Publication Type	Research paper
PubMed ID	17267557
PubMed Abstract	Targeted membrane addition is a hallmark of many cellular functions. In the nervous system, modification of synaptic membrane size has a major impact on synaptic function. However, because of the complex shape of neurons and the need to target membrane addition to very small and polarized synaptic compartments, this process is poorly understood. Here, we show that Gtaxin (GTX), a Drosophila t-SNARE (target-soluble N-ethylmaleimide-sensitive factor attachment protein receptor), is required for expansion of postsynaptic membranes during new synapse formation. Mutations in gtx lead to drastic reductions in postsynaptic membrane surface, whereas gtx upregulation results in the formation of complex membrane structures at ectopic sites. Postsynaptic GTX activity depends on its direct interaction with Discs-Large (DLG), a multidomain scaffolding protein of the PSD-95 (postsynaptic density protein-95) family with key roles in cell polarity and formation of cellular junctions as well as synaptic protein anchoring and trafficking. We show that DLG selectively determines the postsynaptic distribution of GTX to type I, but not to type II or type III boutons on the same cell, thereby defining sites of membrane addition to this unique set of glutamatergic synapses. We provide a mechanistic explanation for selective targeted membrane expansion at specific synaptic junctions.
DOI	10.1523/JNEUROSCI.3160-06.2007
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Neurosci.
Title	Journal of Neuroscience
Publication Year	1981-
ISBN/ISSN	0270-6474 1529-2401
Data from Reference
Aberrations (2)
	Df(1)N71 Df(3R)Exel7357
Alleles (12)
	dlg1⁶ dlg1⁷ dlg1^Scer\UAS.cBa Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^BG487 Scer\GAL4^C57 Scer\GAL4^futsch-C380 Scer\GAL4^T80 Syx18^ex6 Syx18^EY08095 Syx18^Scer\UAS.cGa w^+mC
Constructs (4)
	P{?EPgy2} P{UAS-dlg1.B} P{UAS-mCD8::GFP.L} P{UAS-Syx18.G}
Genes (8)
	dlg1 Mmus\Cd8a Scer\GAL4 scrib Syx1A Syx18 w α-Spec
Insertions (6)
	P{?EPgy2}Syx18^ex6 P{EPgy2}Syx18^EY08095 P{GAL4}BG487 P{GAL4}C380 P{GawB}C57 P{GawB}T80
Natural transposons (1)
	P-element
Transcripts (2)
	Scer\GAL4^BG487RA Scer\GAL4^C57RA