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Reference
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| Citation |
Huisinga, K.L., Figueroa-Clarevega, A., McDaniel, S., Shimonovich, S., Wu, A., Wuller, J., Elgin, S.C. (2007). Repetitious Element 1360 as a Target for Heterochromatin Formation in Drosophila melanogaster. A. Dros. Res. Conf. 48 : 319A.
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| FlyBase ID |
FBrf0197918 |
| Type of publication |
Abstract |
| Offprint Available |
No |
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External Crossreferences
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| PubMed ID |
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| PubMed Abstract |
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| Conference Abstract |
Position effect variegation (PEV) occurs in Drosophila when normally euchromatic genes are relocated to a heterochromatic
domain, either by rearrangement or transposition. This variegating phenotype, which results from silencing of gene expression
in some of the cells where it is normally active, is indicative of heterochromatin formation. Earlier work suggested that
the repetitious element 1360 can serve as an initiation site for heterochromatin formation in the fourth chromosome (Sun et
al. 2004. Mol Cell Biol. 24:8210). To directly test if 1360 is capable of initiating heterochromatin formation, P element
constructs containing either one or four copies of 1360 upstream of an hsp70-white reporter were constructed and transformants
carrying the transgenes were recovered (Haynes et al. 2006 Curr Biol. in press). Additional lines were generated by mobilization
of X-chromosome P element insertions. For each of the 1360-containing P elements 2500 males were screened for mobilization.
With one copy of 1360, 4% of recovered lines exhibit PEV and with four copies of 1360, 12% of recovered lines exhibit PEV.
Previous mobilizations of an hsp70-white P element reporter lacking the 1360 element resulted in a 1% recovery rate of flies
exhibiting PEV (Wallrath & Elgin. 1995. Genes Dev. 9:1263). These results suggest that an increasing number of copies of 1360
increase the likelihood of initiating heterochromatin formation. Interestingly, the percentage of variegating lines recovered
on the fourth chromosome has not increased. Rather, a higher fraction of the variegating lines are now on the second and third
chromosomes. Precise sites of insertion are being mapped to determine if an increase in 1360 copy number allows ectopic heterochromatin
formation outside of the regions normally considered heterochromatic.
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Associated Information
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| Text of personal communication |
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Also Published As
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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Published In
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| Abbreviation |
A. Dros. Res. Conf. 48 |
| Title |
Program and Abstracts. 48th Annual Drosophila Research Conference, Philadelphia, PA, 2007 |
| Authors |
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| Volume range |
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| Year range |
2007 |
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| Publisher |
The Genetics Society of America |
| Place of publication |
Bethesda, MD |
| Language of publication |
English |
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Data from Reference
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| Natural transposons (1)
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