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Gresens, J., Cook, K. (2006.8.29). Isolation and characterization of Df(3R)BSC196. 
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FBrf0198977
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Date: Tue, 29 Aug 2006  15:37:36  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3R)BSC196
Isolation and characterization of Df(3R)BSC196
Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC196 was isolated as a FLP recombinase-induced recombination 
event involving PBac{RB}e01098 and P{XP}CG2747[d09536]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6B, Tb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; PBac{RB}e01098/P{XP}CG2747[d09536] males. The males were 
heat shocked as larvae as described in Parks et al. Nature Genetics 
36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding 
and succeeding generations maintained the original genetic background 
of the Exelixis insertion stocks (Thibault et al., Nature Genetics 
36: 283-287, 2004; FBrf0175002). The recombination event generated 
the genetic element P+PBac{XP5.RB3}BSC196 from the segment of 
PBac{RB}e01098 to the left of its FRT site and the segment of 
P{XP}CG2747[d09536] to the right of its FRT site. The cytological 
breakpoints of Df(3R)BSC196 predicted from the transposable element 
insertions sites using Release 4 coordinates are 84E6;84E8. It failed 
to complement l(3)84Ed[1] and l(3)84Ec[1].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX 
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    English
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    Aberrations (1)
    Alleles (2)
    Genes (2)
    Insertions (3)
    Transgenic Constructs (1)