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Gresens, J., Cook, K. (2006.12.5). Isolation and characterization of Df(3L)BSC220. 
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Date: Tue, 05 Dec 2006  14:55:05  -0500
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3L)BSC220
Cc: Jill Gresens <jgresensXXXX>, Stacey Christensen <sjchristXXXX>, mdealXXXX, kaufmanXXXX
Isolation and characterization of Df(3L)BSC220
Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC220 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}f03118 and P{XP}d09620. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; PBac{WH}f03118/P{XP}d09620 males. The males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC220 from the segment of 
PBac{WH}f03118 to the left of its FRT site and the segment of 
P{XP}d09620 to the right of its FRT site. Its presence was verified 
using the PCR methods and primers described in Parks et al. The 
cytological breakpoints of Df(2L)BSC219 predicted from the Release 4 
genomic coordinates of the transposable element insertions sites 
using are 75F1;76A1. Df(3L)BSC220 failed to complement nkd[2].
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX 
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    Aberrations (1)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)