Isolation and characterization of Df(3R)BSC222
Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC222 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG10435f03495 and P{XP}d08394. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6B females crossed to P{hsFLP}1, y1 w1118; PBac{WH}CG10435f03495/P{XP}d08394 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC222 from the segment of PBac{WH}CG10435f03495 to the left of its FRT site and the segment of P{XP}d08394 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d08394 to be Release 3 genomic coordinate 4076070 on chromosome arm 3R. The Gene Disruption project determined the insertion site of P{XP}d08394 to be Release 3 genomic coordinate 4076158 on arm 3R. This corresponds to 84F6 on both the Release 3 and Release 4 genome maps. The predicted position of PBac{WH}CG10435f03495 on the Release 4 map is 84E8. Consequently, the cytological breakpoints of Df(3R)BSC222 are predicted to be 84E8;84F6. Df(3R)BSC222 failed to complement Tom3403692.