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Christensen, S., Cook, K. (2007.5.8). Isolation and characterization of Df(2L)BSC299. 
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Date: Tue, 08 May 2007  08:45:00  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2L)BSC299
Cc: mdealXXXX, Stacey Christensen <sjchristXXXX>, kaufman@XXXX
Isolation and characterization of Df(2L)BSC299
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC299 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}f03562 and P{XP}d09327. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; PBac{WH}f03562/P{XP}d09327 males crossed to 
w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC299 from the segment of 
PBac{WH}f03562 to the left of its FRT site and the segment of 
P{XP}d09327 to the right of its FRT site. Its presence was verified 
using the PCR methods and primers described in Parks et al. Exelixis, 
Inc. determined the insertion site of PBac{WH}f03562 to be at Release 
3 genomic coordinate 14988104 on chromosome arm 2L. The Gene 
Disruption project determined the insertion site of PBac{WH}f03562 to 
be at Release 3 genomic coordinate 14988091 on arm 2L. This 
corresponds to 35B8 on the Release 5 genome map. The predicted 
position of P{XP}d09327 on the Release 5 map is 35C1. Consequently, 
the cytological breakpoints of Df(2L)BSC299 are predicted to be 
35B8;35C1. It failed to complement l(2)35Bg[2], Su(H)[1], vas[1] and ck[13].
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX 
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    Aberrations (1)
    Alleles (4)
    Genes (4)
    Insertions (3)
    Transgenic Constructs (2)