Isolation and characterization of Df(2L)BSC299
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC299 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f03562 and P{XP}d09327. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}f03562/P{XP}d09327 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC299 from the segment of PBac{WH}f03562 to the left of its FRT site and the segment of P{XP}d09327 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PBac{WH}f03562 to be at Release 3 genomic coordinate 14988104 on chromosome arm 2L. The Gene Disruption project determined the insertion site of PBac{WH}f03562 to be at Release 3 genomic coordinate 14988091 on arm 2L. This corresponds to 35B8 on the Release 5 genome map. The predicted position of P{XP}d09327 on the Release 5 map is 35C1. Consequently, the cytological breakpoints of Df(2L)BSC299 are predicted to be 35B8;35C1. It failed to complement l(2)35Bg2, Su(H)1, vas1 and ck13.