Isolation and characterization of Df(2R)BSC264
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC264 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG1707f04769 and P{XP}d03130. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}CG1707f04769/P{XP}d03130 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC264 from the segment of PBac{WH}CG1707f04769 to the left of its FRT site and the segment of P{XP}d03130 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d03130 to be at Release 3 genomic coordinate 2550729 on chromosome arm 2R. The Gene Disruption project determined the insertion site of P{XP}d03130 to be at Release 3 genomic coordinate 2550735 on arm 2R. This corresponds to 43D1 on the Release 3 genome map and 43C5 on the Release 4 genome map. The predicted position of PBac{WH}CG1707f04769 on the Release 4 map is 43B2. Consequently, the Release 4 cytological breakpoints of Df(2R)BSC264 are predicted to be 43B2;43C5. It failed to complement Aldh-III03610 and soD.