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Christensen, S., Cook, K. (2007.3.22). Isolation and characterization of Df(2R)BSC264. 
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FBrf0199010
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Date: Thu, 22 Mar 2007  14:50:43  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2R)BSC264
Isolation and characterization of Df(2R)BSC264
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC264 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}CG1707[f04769] and P{XP}d03130. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; PBac{WH}CG1707[f04769]/P{XP}d03130 males 
crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC264 from the segment 
of PBac{WH}CG1707[f04769] to the left of its FRT site and the segment 
of P{XP}d03130 to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
Exelixis, Inc. determined the insertion site of P{XP}d03130 to be at 
Release 3 genomic coordinate 2550729 on chromosome arm 2R. The Gene 
Disruption project determined the insertion site of P{XP}d03130 to be 
at Release 3 genomic coordinate 2550735 on arm 2R. This corresponds 
to 43D1 on the Release 3 genome map and 43C5 on the Release 4 genome 
map. The predicted position of PBac{WH}CG1707[f04769] on the Release 
4 map is 43B2. Consequently, the Release 4 cytological breakpoints of 
Df(2R)BSC264 are predicted to be 43B2;43C5. It failed to complement 
Aldh-III[03610] and so[D].
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    English
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    Aberrations (1)
    Alleles (2)
    Genes (2)
    Insertions (3)
    Transgenic Constructs (2)