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Christensen, S., Cook, K. (2007.5.8). Isolation and characterization of Df(2L)BSC290. 
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FBrf0199015
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From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	mdealXXXX, Stacey Christensen <sjchristXXXX>, kaufman@XXXX
Subject: 	Isolation and characterization of Df(2L)BSC290
Date: 	Tue, 08 May 2007  08:46:14  -0400  ( 13:46  BST)
Isolation and characterization of Df(2L)BSC290
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC290 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}f02059 and P{XP}d08229. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; PBac{WH}f02059/P{XP}d08229 males crossed to 
w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC290 from the segment of 
PBac{WH}f02059 to the left of its FRT site and the segment of 
P{XP}d08229 to the right of its FRT site. Its presence was verified 
using the PCR methods and primers described in Parks et al. Exelixis, 
Inc. determined the insertion site of P{XP}d08229 to be at Release 3 
genomic coordinate 13049022 on chromosome arm 2L. This corresponds to 
34A5 on the Release 5 genome map. The predicted position of 
PBac{WH}f02059 on the Release 5 map is 33F2 . Consequently, the 
cytological breakpoints of Df(2L)BSC290 are predicted to be 
33F2;34A5. It failed to complement Vha68-2[s4214] and Df(2L)ED778
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    English
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    Aberrations (2)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)