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Citation
Gregory, S.L., Shandala, T., O'Keefe, L., Jones, L., Murray, M.J., Saint, R. (2007). A Drosophila overexpression screen for modifiers of Rho signalling in cytokinesis.  Fly 1(1): 13--22.
FlyBase ID
FBrf0199094
Publication Type
Research paper
Abstract

To identify genes that modulate Rho signalling during cytokinesis we tested the effect of overexpressing a set of 2190 genes on an eye phenotype caused by defective Rho activation. The resulting 112 modifier loci fell into three main classes: cell cycle genes, signalling effectors and metabolic enzymes. We developed a further series of genetic tests to refine the interactors into those most likely to modify Rho signalling during cytokinesis. In addition to a number of genes previously implicated in the Rho pathway during cytokinesis, we identified four novel primary candidates: cdc14, Pitslre, PDK1 and thread/diap1. cdc14 orthologs have, however, been implicated in cytokinesis in other organisms, as have molecules related to Thread/Diap1. The identification of several modifiers that are genetically redundant paralogs highlights the ability of overexpression screens to identify genes that are refractory to traditional loss-of-function approaches. Overexpression screens and sensitized phenotypes, therefore, may help identify the many factors that are expected to be involved in cytokinesis but have not been discovered by previous genetic screens.

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Related Publication(s)
Personal communication to FlyBase

Re: GS2038.
Aigaki, 2007.3.19, Re: GS2038. [FBrf0199125]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Fly
    Title
    Fly
    Publication Year
    2007-
    ISBN/ISSN
    1933-6934 1933-6942
    Data From Reference
    Alleles (183)
    Genes (137)
    Insertions (157)
    Transgenic Constructs (11)