Reference Report

Reference
Citation	Gregory, S.L., Shandala, T., O'Keefe, L., Jones, L., Murray, M.J., Saint, R. (2007). A Drosophila overexpression screen for modifiers of Rho signalling in cytokinesis. Fly 1(1): 13--22. (Export to RIS)
FlyBase ID	FBrf0199094
Publication Type	Research paper
External Crossreferences
PubMed ID	18690061
PubMed Abstract	To identify genes that modulate Rho signalling during cytokinesis we tested the effect of overexpressing a set of 2190 genes on an eye phenotype caused by defective Rho activation. The resulting 112 modifier loci fell into three main classes: cell cycle genes, signalling effectors and metabolic enzymes. We developed a further series of genetic tests to refine the interactors into those most likely to modify Rho signalling during cytokinesis. In addition to a number of genes previously implicated in the Rho pathway during cytokinesis, we identified four novel primary candidates: cdc14, Pitslre, PDK1 and thread/diap1. cdc14 orthologs have, however, been implicated in cytokinesis in other organisms, as have molecules related to Thread/Diap1. The identification of several modifiers that are genetically redundant paralogs highlights the ability of overexpression screens to identify genes that are refractory to traditional loss-of-function approaches. Overexpression screens and sensitized phenotypes, therefore, may help identify the many factors that are expected to be involved in cytokinesis but have not been discovered by previous genetic screens.
BIOSIS ID	2008.462280
Zoological Record ID
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FB2012_01	Genes GS9605
FB2011_10
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Associated Information
Comments
Text of Personal Communication
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Related Publications
Personal communication to FlyBase	Re: GS2038. Aigaki, 2007.3.19, Re: GS2038. [FBrf0199125]
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Secondary IDs
Language of Publication	English
Additional Languages of Abstract
ISBN
Parent Publication
Publication Type	Journal
Abbreviation	Fly
Title	Fly
Editors
Publication Year	2007-
ISBN/ISSN	1933-6934 1933-6942
Data from Reference
Alleles (183)
	Ack^GS3234 amn^GS1166 apt^GS9203 apt^GS10120 Atu^GS5038 aur^GS7173 B4^GS10012 BacA\p35^Scer\UAS.cHa ball^GS3052 CalpA^GS9103 Cam^GS2043 Cam^GS9113 Cam^GS9963 cdc14^GS9041 Cdk4^GS2301 cenB1A^GS6002 Cerk^GS9104 CG1826^GS1030 CG3036^GS5144 CG3603^GS1133 CG4068^GS1194 CG6701^GS2275 CG8317^GS2301 CG8419^GS2278 CG8451^GS2278 CG10222^GS3108 CG11703^GS3184 CG13917^GS10086 CG14059^GS10553 CG15625^GS5144 CG17283^GS3141 CG30016^GS9676 CG32676^GS1035 CG32812^GS7367 CHES-1-like^GS1023 chrb^GS26 coro^GS2090 coro^GS2249 coro^GS9025 crol^GS2267 Csp^GS9167 Ctf4^GS2275 cv-2^GS9538 CycB3^L6540 CycB² CycH^GS9043 Cyp6v1^GS1124 Dad^GS7178 dia^GS2135 dia^unspecified dl^GS5251 Dlc90F^GS3099 Dlc90F^GS10880 Dp1^GS9049 E2f^GS9647 east^GS1211 Fit1^GS3234 flfl^GS8059 flfl^GS9037 flr^GS3108 fng^GS9108 for^GS9005 for^GS9582 fray^GS3153 fs(1)h^GS1147 fs(1)h^GS5099 Fur1^GS9492 fwd^GS7301 fz4^GS7412 fz4^GS7425 gish^GS10076 Gr5a^GS7438 grp^GS5044 grp^GS6056 GS9605^GS9605 Gα73B^GS10165 Gα73B^GS13032 His2Av^GS3052 Ide^GS51 imd^GS9049 iPLA2-VIA^GS10408 jing^GS2136 jing^GS7350 jing^GS7386 jing^GS9070 kis^GS9968 klg^GS10056 klg^GS10439 Lk6^GS10110 lola^GS5061 Mbs^GS13173 Men^GS9039 Men-b^GS8066 mir-14^GS2072 mir-14^GS7322 mir-14^GS9193 mir-313^GS5097 Mlc2^GS9997 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Mnn1^GS9609 Mob2^GS5030 Mob2^GS8113 Moe^GS1014 Moe^GS6024 mura^GS5193 mura^GS7244 Myo31DF^GS2116 Myo31DF^GS5068 Myo31DF^GS9673 mys::tor^D.Scer\UAS nemy^GS10870 nuf^GS9035 numb^GS7034 ovo^GS31 ovo^GS42 ovo^GS1144 ovo^GS1153 ovo^GS1156 ovo^GS1160 ovo^GS1163 ovo^GS1164 pav^B200 pbl^{dsRNA.Scer\UAS} pbl^Scer\UAS.cPa pbl^{ΔDH497-549.Scer\UAS} Pdk1^GS3218 Pdk1^GS6007 Pdk1^GS6021 Pdk1^GS6026 Pdk1^GS6027 Pdk1^GS10114 Pdk1^GS10133 Pfrx^GS7393 pigs^GS1067 PIP5K59B^GS2580 Pitslre^GS10080 Ptp4E^GS1194 Ptpmeg^GS9137 pum^GS9231 R^GS10082 Rac2^GS10622 Rapgap1^GS9003 ras^GS1035 rdgB^GS1086 rdgB^GS5203 rdgB^GS7372 rdgB^GS7374 rdgB^GS7387 Rho1^72R RhoBTB^GS51 robl37BC^GS2294 Rop^GS10103 RpL30^GS2294 Rrp1^GS9013 sca^GS2038 sca^GS10020 Scer\GAL4^CY2 Scer\GAL4^en-e16E Scer\GAL4^GMR.PF sec63^GS3104 shn^GS2029 shot^GS10458 simj^GS9977 sip1^GS10088 spi^GS9657 Src64B^GS9618 stg^GS3120 sti^GS9053 Sur-8^GS3141 tau^GS10019 tefu^GS13617 th^GS9038 tlk^GS1207 trbl^GS9628 trem^GS10527 ttk^GS3069 ttk^GS3165 vib^GS3184 Vps13^GS78 wdb^GS3071 wdb^GS9684 Wnt5^GS1192 wun^GS10009
Constructs (11)
	P{GAL4-ninaE.GMR} P{GSV1} P{GSV2} P{GSV3} P{GSV6} P{UAS-mCD8::GFP.L} P{UAS-mys::tor.D} P{UAS-p35.H} P{UAS-pbl.1A} P{UAS-pbl.dsRNA} P{UAS-Pbl.ΔDH497-549}
Genes (137)
	Ack amn apt Atu aur B4 BacA\p35 ball CalpA Cam cdc14 Cdk4 cenB1A Cerk CG1826 CG3036 CG3603 CG4068 CG6701 CG8317 CG8419 CG8451 CG10222 CG11703 CG13917 CG14059 CG15625 CG17283 CG30016 CG30499 CG31529 CG32676 CG32812 CHES-1-like chrb coro crol Csp Ctf4 cv-2 CycB CycB3 CycH Cyp6v1 Dad dia dl Dlc90F Dp1 E2f east Fit1 flfl flr fng for fray fs(1)h Fur1 fwd fz4 gish Gr5a grp GS9605 Gα73B His2Av Ide imd iPLA2-VIA jing kis klg Lk6 lola Mbs Men Men-b mir-14 mir-313 Mlc2 Mmus\Cd8a Mnn1 Mob2 Moe mura Myo31DF mys::tor nemy nuf numb ovo pav pbl Pdk1 Pfrx pigs PIP5K59B Pitslre Ptp4E Ptpmeg pum R Rac2 Rapgap1 ras rdgB Rho1 RhoBTB robl37BC Rop RpL30 Rrp1 sca Scer\GAL4 sec63 shn shot simj sip1 spi Src64B stg sti Sur-8 tau tefu th tlk trbl trem ttk vib Vps13 wdb Wnt5 wun
Insertions (157)
	P{en2.4-GAL4}e16E P{GawB}CY2 P{GSV1}Ack^GS3234 P{GSV1}amn^GS1166 P{GSV1}Cam^GS2043 P{GSV1}Cdk4^GS2301 P{GSV1}CG1826^GS1030 P{GSV1}CG3603^GS1133 P{GSV1}CG6701^GS2275 P{GSV1}CG8451^GS2278 P{GSV1}CG11703^GS3184 P{GSV1}CHES-1-like^GS1023 P{GSV1}chrb^GS26 P{GSV1}coro^GS2090 P{GSV1}coro^GS2249 P{GSV1}crol^GS2267 P{GSV1}Cyp6v1^GS1124 P{GSV1}dia^GS2135 P{GSV1}Dlc90F^GS3099 P{GSV1}east^GS1211 P{GSV1}flr^GS3108 P{GSV1}fray^GS3153 P{GSV1}fs(1)h^GS1147 P{GSV1}His2Av^GS3052 P{GSV1}Ide^GS51 P{GSV1}jing^GS2136 P{GSV1}mir-14^GS2072 P{GSV1}Moe^GS1014 P{GSV1}Myo31DF^GS2116 P{GSV1}ovo^GS31 P{GSV1}ovo^GS42 P{GSV1}ovo^GS1144 P{GSV1}ovo^GS1153 P{GSV1}ovo^GS1156 P{GSV1}ovo^GS1160 P{GSV1}ovo^GS1163 P{GSV1}ovo^GS1164 P{GSV1}Pdk1^GS3218 P{GSV1}pigs^GS1067 P{GSV1}PIP5K59B^GS2580 P{GSV1}Ptp4E^GS1194 P{GSV1}ras^GS1035 P{GSV1}rdgB^GS1086 P{GSV1}RpL30^GS2294 P{GSV1}sca^GS2038 P{GSV1}sec63^GS3104 P{GSV1}shn^GS2029 P{GSV1}stg^GS3120 P{GSV1}Sur-8^GS3141 P{GSV1}tlk^GS1207 P{GSV1}ttk^GS3069 P{GSV1}ttk^GS3165 P{GSV1}Vps13^GS78 P{GSV1}wdb^GS3071 P{GSV1}Wnt5^GS1192 P{GSV2}Atu^GS5038 P{GSV2}aur^GS7173 P{GSV2}CG3036^GS5144 P{GSV2}CG32812^GS7367 P{GSV2}Dad^GS7178 P{GSV2}dl^GS5251 P{GSV2}fs(1)h^GS5099 P{GSV2}fwd^GS7301 P{GSV2}fz4^GS7412 P{GSV2}fz4^GS7425 P{GSV2}Gr5a^GS7438 P{GSV2}grp^GS5044 P{GSV2}jing^GS7350 P{GSV2}jing^GS7386 P{GSV2}lola^GS5061 P{GSV2}mir-14^GS7322 P{GSV2}mir-313^GS5097 P{GSV2}Mob2^GS5030 P{GSV2}mura^GS5193 P{GSV2}mura^GS7244 P{GSV2}Myo31DF^GS5068 P{GSV2}numb^GS7034 P{GSV2}Pfrx^GS7393 P{GSV2}rdgB^GS5203 P{GSV2}rdgB^GS7372 P{GSV2}rdgB^GS7374 P{GSV2}rdgB^GS7387 P{GSV3}cenB1A^GS6002 P{GSV3}flfl^GS8059 P{GSV3}grp^GS6056 P{GSV3}Men-b^GS8066 P{GSV3}Mob2^GS8113 P{GSV3}Moe^GS6024 P{GSV3}Pdk1^GS6007 P{GSV3}Pdk1^GS6021 P{GSV3}Pdk1^GS6026 P{GSV3}Pdk1^GS6027 P{GSV6}apt^GS9203 P{GSV6}apt^GS10120 P{GSV6}B4^GS10012 P{GSV6}CalpA^GS9103 P{GSV6}Cam^GS9113 P{GSV6}Cam^GS9963 P{GSV6}cdc14^GS9041 P{GSV6}Cerk^GS9104 P{GSV6}CG13917^GS10086 P{GSV6}CG14059^GS10553 P{GSV6}CG30016^GS9676 P{GSV6}coro^GS9025 P{GSV6}Csp^GS9167 P{GSV6}cv-2^GS9538 P{GSV6}CycH^GS9043 P{GSV6}Dlc90F^GS10880 P{GSV6}Dp1^GS9049 P{GSV6}E2f^GS9647 P{GSV6}flfl^GS9037 P{GSV6}fng^GS9108 P{GSV6}for^GS9005 P{GSV6}for^GS9582 P{GSV6}Fur1^GS9492 P{GSV6}gish^GS10076 P{GSV6}GS9605^GS9605 P{GSV6}Gα73B^GS10165 P{GSV6}Gα73B^GS13032 P{GSV6}iPLA2-VIA^GS10408 P{GSV6}jing^GS9070 P{GSV6}kis^GS9968 P{GSV6}klg^GS10056 P{GSV6}klg^GS10439 P{GSV6}Lk6^GS10110 P{GSV6}Mbs^GS13173 P{GSV6}Men^GS9039 P{GSV6}mir-14^GS9193 P{GSV6}Mlc2^GS9997 P{GSV6}Mnn1^GS9609 P{GSV6}Myo31DF^GS9673 P{GSV6}nemy^GS10870 P{GSV6}nuf^GS9035 P{GSV6}Pdk1^GS10114 P{GSV6}Pdk1^GS10133 P{GSV6}Pitslre^GS10080 P{GSV6}Ptpmeg^GS9137 P{GSV6}pum^GS9231 P{GSV6}R^GS10082 P{GSV6}Rac2^GS10622 P{GSV6}Rapgap1^GS9003 P{GSV6}Rop^GS10103 P{GSV6}Rrp1^GS9013 P{GSV6}sca^GS10020 P{GSV6}shot^GS10458 P{GSV6}simj^GS9977 P{GSV6}sip1^GS10088 P{GSV6}spi^GS9657 P{GSV6}Src64B^GS9618 P{GSV6}sti^GS9053 P{GSV6}tau^GS10019 P{GSV6}tefu^GS13617 P{GSV6}th^GS9038 P{GSV6}trbl^GS9628 P{GSV6}trem^GS10527 P{GSV6}wdb^GS9684 P{GSV6}wun^GS10009