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Reference Report
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| Citation | Estrada, B., Gisselbrecht, S.S., Michelson, A.M. (2007). Perdido encodes a component of a protein complex required for muscle guidance in Drosophila embryonic muscles. A. Dros. Res. Conf. 48 : 15. (Export to RIS) | ||
| FlyBase ID | FBrf0199168 | ||
| Publication Type | Abstract | ||
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| PubMed Abstract | |||
| Conference Abstract | The molecular mechanisms underlying muscle guidance and attachment to tendon cells are poorly understood both in vertebrates
and in Drosophila. We have identified a novel gene, expressed in a subset of embryonic founder myoblasts, that is essential
for Drosophila muscles to find their attachment sites; thus, we have named it perdido (“lost” in Spanish; perd).
It encodes a conserved single-pass transmembrane cell adhesion protein that contains laminin globular extracellular domains
and a small intracellular domain with a PDZ binding consensus sequence at the C-terminus. In vivo visualization of muscle
guidance in both perd mutant embryos and dsRNA-injected embryos show that some ventral muscles fail to reach their attachment
sites and instead form rounded multinucleated myotubes. Genetic interaction experiments done with a newly developed RNA interference
assay combined with in vivo visualization of muscle development suggest that perd may be a ligand for the laminin binding
<font face=symbol>a</font>PS1-<font face=symbol>b</font>PS integrin heterodimer, which is expressed in the tendon cells. We
also have genetic and biochemical evidence indicating that perd is necessary to localize the muscle guidance PDZ protein DGrip
to the plasma membrane. These results suggest that Perd forms an essential protein complex for muscle guidance by engaging
the myotube with the tendon cell via an extracellular interaction with the tendon integrin complex and by an intracellular
interaction with DGrip. The function of perd in muscle pathfinding resembles the role of its vertebrate orthologs NG2/MCSP
in cell migration in the nervous system. |
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| Language of Publication | English | ||
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Parent Publication
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| Publication Type | Compendium | ||
| Abbreviation | A. Dros. Res. Conf. 48 | ||
| Title | Program and Abstracts. 48th Annual Drosophila Research Conference, Philadelphia, PA, 2007 | ||
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| Publication Year | 2007 | ||
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Data from Reference
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| Genes (4)
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