Reference Report

Reference
Citation	Sweeney, N.T., Brenman, J.E., Jan, Y.N., Gao, FB. (2006). The coiled-coil protein shrub controls neuronal morphogenesis in Drosophila. Curr. Biol. 16(10): 1006--1011. (Export to RIS)
FlyBase ID	FBrf0199197
Publication Type	Research paper
PubMed ID	16713958
PubMed Abstract	The diversity of neuronal cells, especially in the size and shape of their dendritic and axonal arborizations, is a striking feature of the mature nervous system. Dendritic branching is a complex process, and the underlying signaling mechanisms remain to be further defined at the mechanistic level. Here we report the identification of shrub mutations that increased dendritic branching. Single-cell clones of shrub mutant dendritic arborization (DA) sensory neurons in Drosophila larvae showed ectopic dendritic and axonal branching, indicating a cell-autonomous function for shrub in neuronal morphogenesis. shrub encodes an evolutionarily conserved coiled-coil protein homologous to the yeast protein Snf7, a key component in the ESCRT-III (endosomal sorting complex required for transport) complex that is involved in the formation of endosomal compartments known as multivesicular bodies (MVBs). We found that mouse orthologs could substitute for Shrub in mutant Drosophila embryos and that loss of Shrub function caused abnormal distribution of several early or late endosomal markers in DA sensory neurons. Our findings demonstrate that the novel coiled-coil protein Shrub functions in the endosomal pathway and plays an essential role in neuronal morphogenesis.
DOI
Related Publication(s)
Supplementary material	The coiled-coil protein shrub controls neuronal morphogenesis in Drosophila. [FBrf0199198]
Review	Neuronal dendrites need an ESCRT. Kruger, 2006, Cell 125(7): 1212 [FBrf0199083]
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Aberrations (2)
	Df(2R)Np5 In(2LR)w45-32n
Alleles (18)
	Mmus\Chmp4b^Scer\UAS.cSa Mmus\Chmp4c^Scer\UAS.cSa Rab4^{Scer\UAS.T:Disc\RFP} Rab5^{Scer\UAS.T:Avic\GFP-EGFP} Rab7^{Scer\UAS.T:Avic\GFP} Scer\GAL4^109(2)80 Scer\GAL4²²¹ Scer\GAL4⁴⁷⁷ Scer\GAL4^elav-C155 Scer\GAL4^Kr.PM Scer\GAL4^αTub84B.PL shrb⁴ shrb^{dsRNA.Scer\UAS} shrb^EY05194 shrb^k11201 shrb^Scer\UAS.cSa shrb^{Scer\UAS.T:Avic\GFP} spin^{Scer\UAS.T:Hcri\RFP}
Constructs (13)
	P{EPgy2} P{GAL4-Kr.C} P{lacW} P{tubP-GAL4} P{UAS-GFP-Rab5} P{UAS-Mmus\Chmp4b.S} P{UAS-Mmus\Chmp4c.S} P{UAS-Rab4-mRFP} P{UAS-Rab7.GFP} P{UAS-shrb.dsRNA} P{UAS-shrb.S} P{UAS-shrb-GFP} P{UAS-spin-RFP.S}
Genes (8)
	Mmus\Chmp4b Mmus\Chmp4c Rab4 Rab5 Rab7 Scer\GAL4 shrb spin
Insertions (6)
	P{EPgy2}shrb^EY05194 P{GawB}109(2)80 P{GawB}221 P{GawB}477 P{GawB}elav^C155 P{lacW}shrb^k11201
Natural transposons (1)
	P-element