Isolation and characterization of Df(3L)BSC223
Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC223 was isolated as a FLP recombinase-induced recombination event involving P{XP}mubd09454 and PBac{WH}f00362. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C,Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}mubd09454/PBac{WH}f00362 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC223 from the segment of P{XP}mubd09454 to the left of its FRT site and the segment of PBac{WH}f00362 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC223 predicted from the Release 4 genomic coordinates of the transposable element insertions sites using are 79A3;79B3. Df(3L)BSC223 failed to complement RpLP001544.