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Christensen, S., Gresens, J., Cook, K. (2007.3.22). Isolation and characterization of Df(3R)BSC179. 
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FBrf0199242
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Personal communication to FlyBase
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Text of Personal Communication
Date: Thu, 22 Mar 2007  14:51:56  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3R)BSC179
Cc: Stacey Christensen <sjchristXXXX>, mdealXXXX, kaufman@XXXX
Isolation and characterization of Df(3R)BSC179
Stacey Christensen, Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC179 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}f01774 and P{XP}d10839. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; P{hs-hid}3, Dr[1]/TM6B, Tb[1] females crossed to P{hsFLP}1, 
y[1] w[1118]; PBac{WH}f01774/P{XP}d10839 males. The males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC179 from the segment of 
PBac{WH}f01774 to the left of its FRT site and the segment of 
P{XP}d10839 to the right of its FRT site. Its presence was verified 
using the PCR methods and primers described in Parks et al. The 
cytological breakpoints of Df(3R)BSC179 predicted from the Release 4 
genomic coordinates of the transposable element insertions sites are 
83A6;83B2.  Df(3R)BSC179 failed to complement Df(3R)Exel6144 and 
Itp-r83A[05616].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX 
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    English
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    Aberrations (2)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)