Date: Wed, 28 Feb 2007 15:33:24 +0900 To: rd120@XXXX From: Yuji Kageyama <kageyama@XXXX> Subject: Re: Helping FlyBase: ADRC-60426 Cc: s-inagakXXXX, ta-kondoXXXX Dear Rachel, Thank you for e-mail. Regarding annotations for MRE genes, we need your help to add some description to Flybase. MRE31 and MRE32 are annotated as CR30009 and CR32730 in Flybase (please cite Inagaki et al. [Genes. Cells, 2005, 10 (12), 1163-1173] as a reference). Since MRE are arbitrary name and we do not have any data on their function (we only published their expression patterns during embryogenesis), it may be better to handle MRE31 and MRE32 as synonyms of these CR entries. MRE3 is also annotated as pncr013:4 , based on Tupy et al. [Proc Natl Acad Sci U S A, 2005, 102(15), 5495-5500]. Since they published 6 mo. earlier than we, MRE3 should be a synonym of pncr013:4 . For MRE16 and other MREs, EST sequences have already been registered in GenBank. Therefore, instead of sequence deposition to GenBank, we would like to register them as new entries into Flybase. Would you tell us how to add new entries to Flybase? For example, as shown below, if we submit a gene name, corresponding EST clone name and the reference, is it enough to register new genes? MRE1 GH22170 MRE2 GH23165 MRE3/ pncr013:4 GM01028 MRE4 GM01267 MRE5 GM03914 MRE6 GM04458 MRE7 GM05777 MRE8 GM07040 MRE9 GM07660 MRE10 GM08676 MRE11 GM09444 MRE12 GM09534 MRE13 GM09828 MRE14 GM10545 MRE15 GM13095 MRE16 GM31309 MRE17 HL01663 MRE18 HL05775 MRE19 LD03248 MRE20 LD07701 MRE21 LD11130 MRE22 LD33458 MRE23 RE65620 MRE24 RH66459 MRE25 RH66770 MRE26 SD04448 MRE27/CR31972 GH10270 MRE28/CR32646 HL02010 MRE29/CR33327/ pncr001:3R LD11162 MRE30/CR32028 LD13080 MRE31/CR30009 RE30084 MRE32/CR32730 RE54930 MRE33/CR31808 RE70695 * Modfied from Inagaki et al., Genes. Cells, 2005, 10 (12), 1163-1173, Table 2) Again, since we do not have any data for their function, it may be better to define these genes as CR entries with MRE numbers, similar to MRE31/32. We know our requests are bit complicated and troublesome but we believe these info are helpful for fly people (at least, much better than nothing). And, If you know more appropriate person to handle these stuffs, please let me know. Regards, -- Yuji Kageyama, PhD <kageyama@XXXX> PREST Investigator Japan Science and Technology Agency 4-1-8 Honcho Kawaguchi, Saitama 332-0012 Assistant Professor Graduate School of Biological Sciences Nara Institute of Science and Technology 8916-5 Takayama Ikoma, Nara 630-0192, Japan phone: +81-743-72-5552 fax: +81-743-72-5559 >From: "Rachel Drysdale (Genetics)" <rd120@XXXX> >Date: 2007G/27n26F| 5:56:46:JST >To: s-inagak@XXXX >Subject: Helping FlyBase: ADRC-60426 > >Dear Sachi, > >We are currently curating the abstracts for the upcoming 48th >(Philadelphia) Annual Drosophila Research Conference, for FlyBase. >I am writing in connection with your abstract: >Characterization and functional analysis of mRNA-like non-coding RNAs in >Drosophila. > >The abstracts mention four interesting genes new to FlyBase - MRE3, >MRE16, MRE31 and MRE32 - which I will enter into FlyBase with those >symbols and the full names mRNA-like ncRNA in embryogenesis 3, 16, 31 >and 32 respectively. > >I am presuming that these genes are not yet described in terms of the >gene models. If you have not already done so I would urge you to >submit the sequences of these genes to DDBJ/EMBL/GenBank, so that they >will eventually be incorporated into the genome annotation. > >Best regards, > >Rachel. > >~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ >Rachel Drysdale. > >FlyBase (Cambridge), >Department of Genetics, >University of Cambridge, >Downing Street, email: rd120@XXXX >Cambridge, CB2 3EH, Ph : 01223-333963 >UK. FAX: 01223-333992 > >http://flybase.org/ >http://www.gen.cam.ac.uk/Research/flybase.htm >~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~