Isolation and characterization of Df(2L)BSC169
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC169 was isolated as a FLP recombinase-induced recombination event involving P{XP}CG6907d06812 and PBac{WH}CG14007f07713. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}CG6907d06812/PBac{WH}CG14007f07713 males crossed to w1118; P{w+mC=hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC169 from the segment of P{XP}CG6907d06812 to the left of its FRT site and the segment of PBac{WH}CG14007f07713 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC169 predicted from the transposable element insertions sites using Release 4 are 25E5;25F3. It failed to complement CG7277KG03584, Hel25Ek11511 and Lam04643.